Tubulin Beta 4 Antibody - Azide and BSA Free

Western Blot: Tubulin Beta 4 Antibody [NBP2-99382] - Anti-TUBB4A rabbit polyclonal antibody at 1:5000 dilution.

Immunocytochemistry/Immunofluorescence: Tubulin Beta 4 Antibody [NBP2-99382] - Immunofluorescence staining of Tubulin Beta 4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99382 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoskeleton.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Tubulin Beta 4 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Tubulin Beta 4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB4A
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Western Blot 1:2000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Tubulin Beta 4 Antibody - Azide and BSA Free

• TUBB4
• TUBB4A
• TUBB5beta-5
• Tubulin 5 beta
• Tubulin Beta 4
• tubulin beta-4 chain
• tubulin, beta 4
• tubulin, beta, 5

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBB4A