TTC11 Antibody (4211) - Azide and BSA Free

Western Blot: TTC11 Antibody (4211) [NBP2-43771] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [4211].

Immunocytochemistry/Immunofluorescence: TTC11 Antibody (4211) [NBP2-43771] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: FIS1 protein stained by FIS1 antibody [4211] diluted at 1:100. Blue: Hoechst 33342 staining.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (NBP2-43771) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: FIS1 protein stained by FIS1 antibody (NBP2-43771) diluted at 1:1000.Red: MitoTrackerR Red CMXRos, a mitochondria tracker.Blue: Hoechst 33342 staining.Scale bar = 10 um.

FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (NBP2-43771) diluted by 1:1000.

• Reactivity: Hu, Rt, Mu, Bv, RM
• Applications: WB, ICC/IF
• Clone: 4211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TTC11 Antibody (4211) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TTC11 Antibody (4211) - Azide and BSA Free (NBP2-43771) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human TTC11 Recombinant protein.
• Predicted Species: Mouse (96%), Rhesus Macaque (100%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FIS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for TTC11 Antibody (4211) - Azide and BSA Free

• CGI-135
• FIS1 homolog
• Fis1
• fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
• fission 1 (mitochondrial outer membrane) homolog (yeast)
• H_NH0132A01.6
• hFis1
• mitochondrial fission 1 protein
• tetratricopeptide repeat domain 11
• Tetratricopeptide repeat protein 11
• TTC11TPR repeat protein 11

Background

The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FIS1