TTBK1 Antibody - BSA Free

Western Blot: TTBK1 Antibody [NBP1-76989] - Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: TTBK1 Antibody - BSA Free [NBP1-76989] - Immunofluorescence of TTBK1 in Human Brain tissue with TTBK1 antibody at 20 ug/mL.

Immunohistochemistry: TTBK1 Antibody - BSA Free [NBP1-76989] - Immunohistochemistry of TTBK1 in mouse brain tissue with TTBK1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TTBK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TTBK1 Antibody - BSA Free (NBP1-76989) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TTBK1. The immunogen is located within amino acids 1190 - 1240 of TTBK1. Amino Acid Squence: DLRPKQPPGRGLGPGR
• Specificity: Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TTBK1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TTBK1 Peptide (NBP1-76989PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TTBK1 Antibody - BSA Free

• BDTK
• Brain-derived tau kinase
• EC 2.7.11
• EC 2.7.11.1
• KIAA1855tau-tubulin kinase 1
• RP3-330M21.4
• tau tubulin kinase 1

Background

Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer's disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients. Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TTBK1
• Uniprot:
  • Q5TCY1(Human)