TSLP Antibody [Unconjugated]

Images

 
Recombinant Human TSLP (Catalog # 1398-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑7 R alpha and TSLP R in a dose-dependent manner (orange line). Proliferation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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TSLP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TSLP
Tyr29-Gln159
Accession # Q969D9
Specificity
Detects human TSLP in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse TSLP and recombinant human TSLP R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TSLP
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.05-0.25 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human TSLP Antibody (Catalog # AF1398)
ELISA Detection:Human TSLP Biotinylated Antibody (Catalog # BAF1398)
Standard: Recombinant Human TSLP (Catalog # 1398-TS)
Reviewed Applications
Read 2 Reviews rated 4
using
AF1398 in the following applications:

Publications
Read Publications using
AF1398 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLP Antibody [Unconjugated]

  • thymic stromal lymphopoietin
  • TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis, and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TSLP Antibody (AF1398)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Antibody Depletion, ELISA Development, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Antibody Depletion
(1)
ELISA Development
(1)
IHC-Fr
(2)
IHC-P
(1)
Neutralization
(4)
Western Blot
(2)
All Applications
Filter By Species
Human
(10)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF1398 Applications Species
B Vizio, M Boita, C Cristiano, J Mazibrada, O Bosco, A Novarino, A Prati, S Sciascia, G Rolla, L Ciuffreda, G Montrucchi, G Bellone Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance Oncotarget, 2018;9(67):32795-32809. 2018 [PMID: 30214685] (Antibody Depletion, Human) Antibody Depletion Human
HL Yang, KK Chang, J Mei, WJ Zhou, LB Liu, L Yao, Y Meng, MY Wang, SY Ha, ZZ Lai, JF Ye, DJ Li, MQ Li Estrogen restricts the apoptosis of endometrial stromal cells by promoting TSLP secretion Mol Med Rep, 2018;0(0):. 2018 [PMID: 30152851] (Neutralization, Human) Neutralization Human
B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils Oncol Lett, 2017;14(6):7483-7488. 2017 [PMID: 29344192] (Neutralization, Human) Neutralization Human
Herro R, Antunes R, Aguilera A, Tamada K, Croft M The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis. J Invest Dermatol, 2015;135(8):2109-18. 2015 [PMID: 25789702] (IHC-Fr, Human) IHC-Fr Human
Kubo T, Kamekura R, Kumagai A, Kawata K, Yamashita K, Mitsuhashi Y, Kojima T, Sugimoto K, Yoneta A, Sumikawa Y, Yamashita T, Sato N, Himi T, Ichimiya S DeltaNp63 controls a TLR3-mediated mechanism that abundantly provides thymic stromal lymphopoietin in atopic dermatitis. PLoS ONE, 2014;9(8):e105498. 2014 [PMID: 25171086] (Neutralization, Human) Neutralization Human
Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells. J Immunol, 0;192(4):1502-11. 0 [PMID: 24453244] (Neutralization, Human) Neutralization Human
Schuepbach-Mallepell S, Philippe V, Bruggen M, Watanabe H, Roques S, Baldeschi C, Gaide O Antagonistic effect of the inflammasome on thymic stromal lymphopoietin expression in the skin. J Allergy Clin Immunol, 2013;132(6):1348-57. 2013 [PMID: 23953709] (IHC-Fr, Mouse) IHC-Fr Mouse
Kashyap M, Rochman Y, Spolski R Thymic stromal lymphopoietin is produced by dendritic cells. J. Immunol., 2011;187(3):1207-11. 2011 [PMID: 21690322] (Western Blot, Human) Western Blot Human
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans. Blood, 2010;116(12):2061-9. 2010 [PMID: 20538796] (Western Blot, Human) Western Blot Human
Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs. Arthritis Rheum., 2008;58(11):3510-9. 2008 [PMID: 18975342] (IHC-P, Human) IHC-P Human
Show All 11 Publications.

Reviews for TSLP Antibody (AF1398) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF1398:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-Fr Human 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 20624642
SpeciesHuman
  4
reviewed by:
Verified Customer
ICC Human 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 23335246
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23335246

Details

Detection NotesSee PMID 23335246

Comments

CommentsPublished in PubMed: PMID 23335246

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Secondary Antibodies

 

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Biotin BAF1398

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Recent Reviews

4
5
0
4
2
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0
2
0
1
0

 
Verified Customer
01/05/2015
Application: IHC-Fr
Species: Human

 
Verified Customer
01/05/2015
Application: ICC
Species: Human

Bioinformatics

Gene Symbol TSLP
Uniprot