TSLP Antibody (258136)

Western blot shows lysates of human lung and kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB1398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 258136
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TSLP Antibody (258136) Summary

• Immunogen: E. coli-derived recombinant human TSLP
  Tyr29-Gln159
  Accession # Q969D9
• Specificity: Detects human TSLP in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑7 or rmTSLP.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSLP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLP Antibody (258136)

• thymic stromal lymphopoietin
• TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1-3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220⁺/IgM^- pre-B cells, mouse TSLP promotes the development B220⁺/IgM⁺ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c⁺ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma _c.

1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
5. Quentmeier, H. et al. (2001) Leukemia 15:1286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSLP Antibody (MAB1398)(2)

Publications using MAB1398

• Abe, S;Harada, N;Sandhu, Y;Sasano, H;Tanabe, Y;Ueda, S;Nishimaki, T;Sato, Y;Takeshige, T;Harada, S;Akiba, H;Takahashi, K; Co-Stimulation with TWEAK and TGF-?1 Induces Steroid-Insensitive TSLP and CCL5 Production in BEAS-2B Human Bronchial Epithelial Cells International journal of molecular sciences 2024-10-29 [PMID: 39519176] (Western Blot, Human)
• Colletti E, Lindstedt S, Park PJ, Almeida-Porada G, Porada CD Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction. Exp. Hematol., 2008-04-08;36(7):816-22. 2008-04-08 [PMID: 18400364] (IHC-Fr, Ovine)

Bioinformatics

• Gene Symbol: TSLP
• Uniprot:
  • Q969D9()