TSLP Antibody (152614)

Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 Ra in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TSLP Monoclonal Antibody (Catalog # MAB555). The ND50 is typically 0.1-0.4 µg/mL.

TSLP neutralization inhibits airway structural alterations.(A) The depth of peribronchial collagen deposition was significantly reduced in anti-TSLP-treated mice, compared with HDM-exposed and IgG isotype-treated controls. (B) Epithelial goblet cells were markedly reduced in anti-TSLP-treated mice, compared to the HDM-exposed and IgG -treated controls. (C) TGF-beta 1 levels in the BALF were significantly increased in HDM-exposed mice. The blockage of TSLP with the anti-TSLP mAb reduced TGF-beta 1levels in the BALF. TGF-beta 1 protein and mRNA levels were determined in duplicate experiments. (D-G) Representative photomicrographs of Masson's trichrome-stained lung sections from mice that were exposed for 5 weeks to saline (D) or HDM (E) and pretreated with anti-TSLP mAb (F) or an IgG-treated control (G) 60 minutes prior to and during HDM exposure. (H-K) Representative photomicrographs of airway sections of PAS-stained from mice that were exposed for 5 weeks to saline (H) or HDM (I) and pretreated with anti-TSLP mAb (J) or an IgG-treated control (K) 60 minutes prior to and during the HDM exposure. Original magnification, 20×. The data shown represent the means±SEM (n = 5), **p<0.01 compared with the HDM group, ##p<0.01 compared with the IgG-treated control mice. The photomicrographs of H-K are representative of 5 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0051268), licensed under a CC-BY license. Not internally tested by R&D Systems.

TSLP neutralization inhibits surface marker expression on airway CD11c+ cells.(A) Lung cell suspensions from mice were stained with combinations of PerCP-Cy 5.5-conjugated CD11c, PE-conjugated OX40L, FITC-conjugated CD80 and APC-conjugated CD86. The dead cells and the debris were excluded based on forward scatter (FSC)/side scatter (SSC) plots. (B) A total of 1×105 to 3×105 viable cells were acquired using FACS, and the DCs were then sorted as CD11c+ airway cells on FSC/SSC plots. (C) Mice that were chronically exposed to HDM received an intranasal administration of anti-TSLP mAb or of a control IgG 60 minutes prior to each HDM exposure. The expression levels of OX40L, CD80 and CD86 on pulmonary DCs were then analyzed using flow cytometry. The HDM-exposed mice exhibited a significant increase in the OX40L, CD80 and CD86 surface markers on CD11c+ airway cells. However, anti-TSLP pretreatment effectively reduced OX40L, CD80 and CD86 expression on the DCs, even though the mice were continuously exposed to HDM. (D) The TSLP levels in the BALF were highly correlated with OX40L expression on CD11c+ airway cells in all of the treatment mice (n = 20, R = 0.879, p<0.01). (E) Representative data from one of the five replicates. (F) Staining for OX40L (12-4031), CD80 (11-4888), and CD86 (17-4321) from isotype-Ig-treated controls. The data represent the means±SEM (n = 5). * p<0.05 or ** p<0.01 compared with the HDM group. # p<0.05 or ## p<0.01 compared with the IgG-treated control mice. The results are derived from 4 experimental groups (5 mice/per group), and the data are representative of 5 independent experiment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23300949), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 152614
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TSLP Antibody (152614) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
  Tyr20-Glu140
  Accession # Q9JIE6
• Specificity: Detects mouse TSLP in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TSLP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSLP Antibody (152614)

• thymic stromal lymphopoietin
• TSLP

Background

TSLP is an IL-7 related cytokine secreted by multiple tissues. It interacts with the heterodimeric receptor complex consisting of IL-7 R alpha and TSLP R. Mouse TSLP stimulates the growth of thymocytes and mature T cells and also supports B cell lymphopoiesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSLP Antibody (MAB555)(25)

Publications using MAB555

• D Sacks, SH Lee, B Kang, O Kamenyeva, T Ferreira, K Cho, J Khillan, J Kabat, B Kelsall Dermis resident macrophages orchestrate localized ILC2-eosinophil circuitries to maintain their M2-like properties and promote non-healing cutaneous leishmaniasis Research square, 2023-04-05;0(0):. 2023-04-05 [PMID: 37066418] (Neutralization, Mouse)
• Z Shen, W Luo, B Tan, K Nie, M Deng, S Wu, M Xiao, X Wu, X Meng, T Tong, C Zhang, K Ma, Y Liao, J Xu, X Wang Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn&#039;s disease EBioMedicine, 2022-09-28;85(0):104285. 2022-09-28 [PMID: 36182776] (In Vivo, Mouse)
• W Yao, B German, D Chraa, A Braud, C Hugel, P Meyer, G Davidson, P Laurette, G Mengus, E Flatter, P Marschall, J Segaud, M Guivarch, P Hener, MC Birling, D Lipsker, I Davidson, M Li Keratinocyte-derived cytokine TSLP promotes growth and metastasis of melanoma by regulating the tumor-associated immune microenvironment JCI Insight, 2022-11-08;0(0):. 2022-11-08 [PMID: 36107619] (Neutralization, Mouse)
• Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su Eosinophil extracellular traps drive asthma progression through neuro-immune signals Nature Cell Biology, 2021-10-06;23(10):1060-1072. 2021-10-06 [PMID: 34616019] (In Vivo, Mouse)
• N Majumder, WT Goldsmith, VK Kodali, M Velayutham, SA Friend, VV Khramtsov, TR Nurkiewicz, A Erdely, PC Zeidler-Er, V Castranova, JR Harkema, EE Kelley, S Hussain Oxidant-induced epithelial alarmin pathway mediates lung inflammation and functional decline following ultrafine carbon and ozone inhalation co-exposure Redox Biology, 2021-08-05;46(0):102092. 2021-08-05 [PMID: 34418598] (Neutralization, Mouse)
• Hea Yon Lee, Hwa Young Lee, Jung Hur, Hye Seon Kang, Joon Young Choi, Chin Kook Rhee et al. Blockade of thymic stromal lymphopoietin and CRTH2 attenuates airway inflammation in a murine model of allergic asthma The Korean Journal of Internal Medicine 2020-05-01 [PMID: 32183504]
• S Li, Z Yi, M Deng, MJ Scott, C Yang, W Li, Z Lei, NM Santerre, P Loughran, TR Billiar TSLP protects against liver I/R injury via activation of the PI3K/Akt pathway JCI Insight, 2019-11-14;4(22):. 2019-11-14 [PMID: 31723054] (Mouse)
• NR Han, PD Moon, HM Kim, HJ Jeong TSLP Exacerbates Septic Inflammation via Murine Double Minute 2 (MDM2) Signaling Pathway J Clin Med, 2019-09-01;8(9):. 2019-09-01 [PMID: 31480519] (Neutralizing, Mouse)
• Sheng-Chieh Lin, Hsiu-Chu Chou, Chung-Ming Chen, Bor-Luen Chiang Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF Pediatric Research 2019-08-01 [PMID: 30464333]
• Verma M, Liu S, Michalec L, Sripada A, Gorska M, Alam R Experimental asthma persists in IL-33 receptor knockout mice because of the emergence of thymic stromal lymphopoietin-driven IL-9&lt;sup&gt;+&lt;/sup&gt; and IL-13&lt;sup&gt;+&lt;/sup&gt; type 2 innate lymphoid cell subpopulations. J Allergy Clin Immunol, 2017-11-10;0(0):. 2017-11-10 [PMID: 29132961] (Neutralization, Mouse)
• Y Cao, E Zhang, J Yang, Y Yang, J Yu, Y Xiao, W Li, D Zhou, Y Li, B Zhao, H Yan, M Lu, M Zhong, H Yan Frontline Science: Nasal epithelial GM-CSF contributes to TLR5-mediated modulation of airway dendritic cells and subsequent IgA response J. Leukoc. Biol., 2017-05-18;0(0):. 2017-05-18 [PMID: 28522600] (Neutralization, Mouse)
• Yahui Hu, Hangming Dong, Mengchen Zou, Chaowen Huang, Lishan Luo, Changhui Yu et al. TSLP signaling blocking alleviates E-cadherin dysfunction of airway epithelium in a HDM-induced asthma model Cellular Immunology 2017-05-01 [PMID: 28400057]
• Critical role of TSLP-responsive mucosal dendritic cells in the induction of nasal antigen-specific IgA response Mucosal Immunol, 2016-12-07;0(0):. 2016-12-07 [PMID: 27924821] (Neutralization, Mouse)
• Thymic Stromal Lymphopoietin Neutralization Inhibits the Immune Adjuvant Effect of Di-(2-Ethylhexyl) Phthalate in Balb/c Mouse Asthma Model PLoS ONE, 2016-07-28;11(7):e0159479. 2016-07-28 [PMID: 27467143] (Neutralization, Mouse)
• Yu M, Owens D, Ghosh S, Farber D Conditional PDK1 Ablation Promotes Epidermal and T-Cell-Mediated Dysfunctions Leading to Inflammatory Skin Disease. J Invest Dermatol, 2015-06-22;135(11):2688-96. 2015-06-22 [PMID: 26099023] (IHC, Mouse)
• Cipolat S, Hoste E, Natsuga K, Quist S, Watt F Epidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility. Elife, 2014-05-05;3(0):e01888. 2014-05-05 [PMID: 24843010] (In Vivo, Mouse)
• Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop. J Invest Dermatol, 2013-11-25;134(5):1295-304. 2013-11-25 [PMID: 24352037] (Neutralization, Mouse)
• Zhuang-Gui Chen, Tian-Tuo Zhang, Hong-Tao Li, Fen-Hua Chen, Xiao-Ling Zou, Jing-Zhi Ji et al. Neutralization of TSLP Inhibits Airway Remodeling in a Murine Model of Allergic Asthma Induced by Chronic Exposure to House Dust Mite PLoS ONE 2013-01-02 [PMID: 23300949]
• Oh MH, Oh SY, Yu J IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin. J. Immunol., 2011-05-16;186(12):7232-42. 2011-05-16 [PMID: 21576506] (Neutralization, Mouse)
• Olkhanud PB, Rochman Y, Bodogai M Thymic stromal lymphopoietin is a key mediator of breast cancer progression. J. Immunol., 2011-04-13;186(10):5656-62. 2011-04-13 [PMID: 21490155] (Neutralization, Mouse)
• Jung K, Tanaka A, Fujita H, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H Peroxisome proliferator-activated receptor gamma-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/Tnd mice. J. Allergy Clin. Immunol., 2011-01-05;127(2):420-29. 2011-01-05 [PMID: 21208653] (ICC, Neutralization, Mouse)
• Miyata M, Nakamura Y, Shimokawa N, Ohnuma Y, Katoh R, Matsuoka S, Okumura K, Ogawa H, Masuyama K, Nakao A Thymic stromal lymphopoietin is a critical mediator of IL-13-driven allergic inflammation. Eur. J. Immunol., 2009-11-01;39(11):3078-83. 2009-11-01 [PMID: 19658093] (Neutralization, Mouse)
• Boehme SA, Franz-Bacon K, Chen EP, Sasik R, Sprague LJ, Ly TW, Hardiman G, Bacon KB A small molecule CRTH2 antagonist inhibits FITC-induced allergic cutaneous inflammation. Int. Immunol., 2008-12-09;21(1):81-93. 2008-12-09 [PMID: 19066314] (Neutralization, Mouse)
• Sokol CL, Barton GM, Farr AG, Medzhitov R A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat. Immunol., 2008-03-01;9(3):310-8. 2008-03-01 [PMID: 18300366] (Flow Cytometry, Mouse)
• Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells. Blood, 2007-01-16;109(9):3706-12. 2007-01-16 [PMID: 17227831] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: TSLP
• Uniprot:
  • Q9JIE6()