TSG101 Antibody (1065916) [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clone: 1065916
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 647

TSG101 Antibody (1065916) [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects TSG101 in direct ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSG101 Antibody (1065916) [Alexa Fluor™ Plus 647]

• ESCRT-I complex subunit TSG101
• TSG10
• TSG101
• tumor susceptibility gene 10
• tumor susceptibility gene 101 protein
• tumor susceptibility gene 101
• tumor susceptibility protein
• VPS23

Background

TSG101 is an essential member of the ESCRT-1 complex which regulates the sorting of ubiquitinated proteins to endosomes, facilitating vesicular trafficking and is implicated in normal development.  It is also involved in regulating transcription, protein sorting, biogenesis of multi-vesicular bodies, and viral budding.  Dysregulation of ESCRT proteins occurs in the development of various human diseases, including many types of cancers and neurodegenerative diseases.  TSG101 is an established cancer-associated gene and truncated aberrantly spliced mRNAs have been reported in various types of cancer. TSG101 is also commonly used as a marker protein for exosomes.

1. Tufan AB, Lazarow K, Kolesnichenko M, Sporbert A, von Kries JP, Scheidereit C. TSG101 associates with PARP1 and is essential for PARylation and DNA damage-induced NF-kappa B activation. EMBO J. 2022 Nov 2;41(21):e110372.
   doi: 10.15252/embj.2021110372. Epub 2022 Sep 20. PMID: 36124865; PMCID: PMC9627669. 
2. Chua HH, Kameyama T, Mayeda A, Yeh TH. Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma. Int J Mol Sci. 2019 Feb 12;20(3):773.
   doi: 10.3390/ijms20030773. PMID: 30759747; PMCID: PMC6387056. 
3. Willms E, Johansson HJ, Mäger I, Lee Y, Blomberg KE, Sadik M, Alaarg A, Smith CI, Lehtiö J, El Andaloussi S, Wood MJ, Vader P. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep. 2016 Mar 2;6:22519.
   doi: 10.1038/srep22519. PMID: 26931825; PMCID: PMC4773763.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.