TSC1 Antibody - BSA Free

Images

 
Western Blot: TSC1 Antibody [NBP1-76618] - Western blot analysis of TSC1 in EL4 cell lysate with anti-TSC1 (IN) using IMG-5539 at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: TSC1 Antibody [NBP1-76618] - EL4 cells with TSC1 antibody at 2 ug/ml
Immunocytochemistry/ Immunofluorescence: TSC1 Antibody [NBP1-76618] - L1210 cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price

TSC1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide from the middle region of human TSC1. The immunogen is located within amino acids 220 - 270 of TSC1. Amino Acid Squence: KDHELDPRRWKRLE
Specificity
At least two isoforms of TSC1 are known to exist; this antibody will detect both isoforms. TSC1 antibody is predicted to not cross-react with TSC2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSC1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Western Blot 1 ug/ml
Application Notes
TSC1 antibody can be used for the detection of TSC1 by Western blot at 1 mg/ml.
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TSC1 Peptide (NBP1-76618PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TSC1 Antibody - BSA Free

  • Hamartin
  • KIAA0243MGC86987
  • LAMhamartin
  • TSC
  • TSC1
  • Tuberous sclerosis 1 protein
  • tuberous sclerosis 1
  • tumor suppressor

Background

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for TSC1 Antibody (NBP1-76618)

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Blogs on TSC1.

TSC1 - a negative regulator of mTOR signaling
TSC1 is a tumor suppressor gene that encodes a 130 kDa protein called hamartin. TSC1 was first identified as an oncogenic driver of Tuberous Sclerosis, a condition characterized by numerous benign tumors of the skin, brain, heart, and lungs. A mut...  Read full blog post.

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Bioinformatics

Gene Symbol TSC1
Uniprot