TSC1 Antibody (SR1192) - BSA Free

Western Blot: TSC1 Antibody (SR1192) [NBP3-22167] - Western blot analysis using NBP3-22167 in HeLa cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SR1192
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TSC1 Antibody (SR1192) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human TSC1 (Uniprot #: Q92574)
• Specificity: Detects endogenous levels of total TSC1
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TSC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:1000-1:5000
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TSC1 Antibody (SR1192) - BSA Free

• Hamartin
• KIAA0243MGC86987
• LAMhamartin
• TSC
• TSC1
• Tuberous sclerosis 1 protein
• tuberous sclerosis 1
• tumor suppressor

Background

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSC1