![Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/Tryptophanyl-tRNA-synthetase-Antibody-Western-Blot-NBP2-32186-img0001.jpg "Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, IHC, IHC-P, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 421 to 471 of human Tryptophanyl-tRNA Synthetase using the numbering given in entry NP_004175.2 (GeneID 7453). |
| Predicted Species | Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | WARS |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for Tryptophanyl tRNA synthetase Antibody (NBP2-32186)Discover more about diseases related to Tryptophanyl tRNA synthetase Antibody (NBP2-32186).
| Pathways for Tryptophanyl tRNA synthetase Antibody (NBP2-32186)View related products by pathway.
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| Gene Symbol | WARS |
| Uniprot |
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![Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize2/Tryptophanyl-tRNA-synthetase-Antibody-Immunohistochemistry-NBP2-32186-img0003.jpg "Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).")
![Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32186 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/Tryptophanyl-tRNA-synthetase-Antibody-Immunoprecipitation-NBP2-32186-img0002.jpg "Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32186] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32186 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32186 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: WARS2 Antibody [NBP1-54653] - Sample Tissue: Human Fetal Muscle, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12](http://images.novusbio.com/images2/WARS2-Antibody-Western-Blot-NBP1-54653-img0004.jpg)
![Western Blot: WARS2 Antibody [NBP1-54653] - Human Fetal Muscle. Lane A: Primary Antibody. Lane B: Primary Antibody and Blocking Peptide.](http://images.novusbio.com/images2/WARS2-Antibody-Western-Blot-NBP1-54653-img0003.jpg)
![Western Blot: WARS2 Antibody [NBP1-54653] - Human Muscle lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/images2/WARS2-Antibody-Western-Blot-NBP1-54653-img0002.jpg)
![Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in control (vector only transfected HEK293T lysate) and TYRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/TYRP1-Antibody-Western-Blot-NBP1-88370-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: TYRP1 Antibody [NBP1-88370] - Staining of human cell line SK-MEL-30 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/TYRP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88370-img0005.jpg)
![Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/images2/TYRP1-Antibody-Immunohistochemistry-Paraffin-NBP1-88370-img0016.jpg)
![Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/images2/TBP like protein TLP Antibody-Western Blot-NBP1-31133-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TBP like protein TLP Antibody [NBP1-31133] - NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLP protein stained by TLP antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/images2/TBP-like-protein-TLP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31133-img0006.jpg)
![Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP1-31133] - Paraffin-embedded mouse lung. TLP antibody dilution: 1:500.](http://images.novusbio.com/images2/TBP-like-protein-TLP-Antibody-Immunohistochemistry-Paraffin-NBP1-31133-img0008.jpg)
![Western Blot: YARS Antibody [NBP1-86889] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-YARS antibody. Remaining relative intensity is presented.](http://images.novusbio.com/images2/YARS-Antibody-Western-Blot-NBP1-86889-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: YARS Antibody [NBP1-86889] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/images2/YARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86889-img0008.jpg)
![Immunohistochemistry-Paraffin: YARS Antibody [NBP1-86889] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-86889 antibody. Corresponding YARS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/YARS-Antibody-Immunohistochemistry-Paraffin-NBP1-86889-img0022.jpg)
![Western Blot: YARS2 Antibody [NBP2-20928] - Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/YARS2 Antibody-Western Blot-NBP2-20928-img0001.jpg)
![Immunohistochemistry-Paraffin: YARS2 Antibody [NBP2-20928] - Human colon carcinoma, using YARS2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/images2/YARS2 Antibody-Immunohistochemistry-Paraffin-NBP2-20928-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Immunohistochemistry-Paraffin-NBP1-81838-img0010.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0011.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0006.jpg)
![SDS-Page: Recombinant Human Gastrin peptide Protein [H00002520-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00002520-P01-1.jpg)
![Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.](http://images.novusbio.com/images2/Catalase-Antibody-Western-Blot-NBP2-24916-img0001.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/images2/Catalase-Antibody-Immunohistochemistry-Paraffin-NBP2-24916-img0002.jpg)
![Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Western-Blot-NBP2-75930-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0007.jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody [NB100-689] - Formalin fixed paraffin embedded colon carcinoma stained with COX-2 antibody (NB100-689).](http://images.novusbio.com/images2/COX-2-Antibody-Immunohistochemistry-Paraffin-NB100-689-img0010.jpg)
![Simple Western: COX-2 Antibody [NB100-689] - Simple Western lane view shows a specific band for COX2 in 1.0 mg/ml of Rat Brain at an antibody concentration of 1:100. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image provided through customer review.](http://images.novusbio.com/images2/COX-2-Antibody-Simple-Western-NB100-689-img0003.jpg)
![Western Blot: QARS Antibody [NBP1-89487] - Analysis using Anti-QARS antibody NBP1-89487 (A) shows similar pattern to independent antibody NBP2-37883 (B).](http://images.novusbio.com/images2/QARS-Antibody-Western-Blot-NBP1-89487-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: QARS Antibody [NBP1-89487] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/images2/QARS-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89487-img0007.jpg)
![Immunohistochemistry-Paraffin: QARS Antibody [NBP1-89487] - Staining of human cerebral cortex.](http://images.novusbio.com/images2/QARS-Antibody-Immunohistochemistry-Paraffin-NBP1-89487-img0013.jpg)
![Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/images2/GLYAT-Antibody-Western-Blot-NBP2-58917-img0002.jpg)
![Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.](http://images.novusbio.com/images2/GLYAT Antibody-Immunohistochemistry-Paraffin-NBP2-58917-img0010.jpg)
![Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/images2/GLYAT-Antibody-Immunohistochemistry-Paraffin-NBP2-58917-img0004.jpg)
![Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Analysis in human testis and lymph node tissues. Corresponding CRAT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CRAT-Antibody-Immunohistochemistry-Paraffin-NBP1-86616-img0014.jpg)
![Western Blot: CRAT Antibody [NBP1-86616] - Analysis using Anti-CRAT antibody NBP1-86616 (A) shows similar pattern to independent antibody NBP1-86615 (B).](http://images.novusbio.com/images2/CRAT-Antibody-Western-Blot-NBP1-86616-img0011.jpg)
![Western Blot: CRAT Antibody [NBP1-86616] - Analysis in human cell line TD47D.](http://images.novusbio.com/images2/CRAT-Antibody-Western-Blot-NBP1-86616-img0010.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/images2/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-24915-img0006.jpg)
![Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of Superoxide Dismutase 1 in FFPE human liver tissue using an isotype control (top) and NBP2-24915 (bottom) at 5 ug/ml.](http://images.novusbio.com/images2/SOD1-Cu-Zn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP2-24915-img0003.jpg)
![Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of SOD in liver lysate of 1) human, 2) mouse, and 3) rat using this antibody. 025 ug/ml.](http://images.novusbio.com/images2/SOD1-Cu-Zn-SOD-Antibody-Western-Blot-NBP2-24915-img0004.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/images2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0010.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0016.jpg)
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Analysis in control (vector only transfected HEK293T lysate) and IDO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Indoleamine-2-3-dioxygenase-IDO-Antibody-Western-Blot-NBP1-87702-img0017.jpg)
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Staining of human lymph node shows strong cytoplasmic and nuclear positivity in non-germinal center cells.](http://images.novusbio.com/images2/Indoleamine-2-3-dioxygenase-IDO-Antibody-Immunohistochemistry-Paraffin-NBP1-87702-img0018.jpg)
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody [NBP1-87702] - Analysis in human cell line EFO-21.](http://images.novusbio.com/images2/Indoleamine-2-3-dioxygenase-IDO-Antibody-Western-Blot-NBP1-87702-img0013.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")