Tryptophanyl tRNA synthetase Antibody - BSA Free

Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- WARS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunoprecipitation: Tryptophanyl tRNA synthetase Antibody [NBP2-32185] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Tryptophanyl tRNA synthetase antibody NBP2-32185 used for IP at 6 ug per reaction. For blotting immunoprecipitated Tryptophanyl tRNA synthetase, NBP2-32185 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Tryptophanyl tRNA synthetase Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Tryptophanyl-tRNA Synthetase using the numbering given in entry NP_004175.2 (GeneID 7453).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WARS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tryptophanyl tRNA synthetase Antibody - BSA Free

• EC 6.1.1
• EC 6.1.1.2
• GAMMA-2
• hWRS
• IFI53
• IFP53trpRS
• Interferon-induced protein 53
• TrpRS
• tryptophan tRNA ligase 1, cytoplasmic
• Tryptophan--tRNA ligase
• tryptophanyl-tRNA synthetase
• tryptophanyl-tRNA synthetase, cytoplasmic
• WRS

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WARS1
• Uniprot:
  • NP_004175.2(Human)