Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [Alexa Fluor® 405] Summary
| Additional Information |
Recombinant Monoclonal Antibody |
| Immunogen |
This antibody was obtained from a rabbit immunized with purified, recombinant Human Tryptophan Hydroxylase 1/TPH-1 (P17752-1; Ile2-Ile444). |
| Isotype |
IgG |
| Clonality |
Monoclonal |
| Host |
Rabbit |
| Gene |
TPH1 |
| Purity |
Protein A purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein A purified |
Notes
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [Alexa Fluor® 405]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Species: Hu
Applications: WB, ICC/IF, PEP-ELISA
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, IHC-FrFl, IHC-WhMt, KO
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: WB, ELISA, EIA, IHC, IHC-P, PEP-ELISA, S-ELISA
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Pm, Bv(-), Ch(-), Rt(-), Ze(-)
Applications: WB, ICC/IF
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Simple Western, IHC, IHC-P
Species: Hu, Pm, Pm
Applications: IHC, IHC-P
Species: Hu
Applications: Flow, IHC, CyTOF-ready
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF
Species: Mu, Rt
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
Species: Hu
Applications: WB
Publications for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405) (0)
There are no publications for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405) (0)
There are no reviews for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405) (0)
Secondary Antibodies
| |
Isotype Controls
|
Other Available Formats
Additional Tryptophan Hydroxylase 1/TPH-1 Products
Bioinformatics Tool for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405)
Discover related pathways, diseases and genes to Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405). Need help?
Read the
Bioinformatics Tool Guide for instructions on using this tool.
Diseases for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405)
Discover more about diseases related to Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405).
| | Pathways for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405)
View related products by pathway.
|
PTMs for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405)
Learn more about PTMs related to Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405).
| | Research Areas for Tryptophan Hydroxylase 1/TPH-1 Antibody (NBP2-90022AF405)
Find related products by research area.
|
Blogs on Tryptophan Hydroxylase 1/TPH-1
![Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [Alexa Fluor® 405]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [Alexa Fluor® 405]")
![Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.](http://images.novusbio.com/images2/Tryptophan-hydroxylase-2-Antibody-Immunohistochemistry-Paraffin-NB100-74555-img0009.jpg)
![Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/Tryptophan-hydroxylase-2-Antibody-Western-Blot-NB100-74555-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan hydroxylase 2-positive double stained cells in the dorsal raphe nuclei. Representative photomicrographs were taken at magnifications of 100x. Quantification of 5-HT and Tryptophan hydroxylase 2. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2218-273X/10/1/71), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Tryptophan-hydroxylase-2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74555-img0011.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Human Colon lysate, 35 ug protein in RIPA buffer. Antibody at 2 ug/mL. Detection by chemiluminescence.](http://images.novusbio.com/images2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-NB100-1892-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [NB100-1892] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/images2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1892-img0005.jpg)
![Knockout Validated: TDO2 Antibody [H00006999-B01P] - Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot. The total protein (20 ug) of the dentate gyrus, extracted from the wild-type (lanes 1 and 3) and Tdo-/- mice (lane 2), was subjected to 4% to 12% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis, and transferred to polyvinylidene difluoride membranes. TDO was detected as described in the Methods section. Note that a 45-kDa band was detected with the anti-TDO antibody. The positions of molecular weight markers are shown on the left. Lane 3: negative control without the primary antibody. beta-Tubulin is a positive control for the Western blot. Image collected and cropped by CiteAb from the following publication (http://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TDO2-Antibody-Western-Blot-H00006999-B01P-img0006.jpg)
![Western Blot: TDO2 Antibody [H00006999-B01P] - Analysis of TDO2 expression in transfected 293T cell line by TDO2 polyclonal antibody. Lane 1: TDO2 transfected lysate(44.66 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/TDO2-Antibody-Western-Blot-H00006999-B01P-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TDO2 Antibody [H00006999-B01P] - Immunoreactivity of TDO in the SVZ, the olfactory bulb, and the cerebellum. In the cerebellum, the immunoreactivity for TDO was observed in granule cells and Purkinje cells of wild type mice, but these positive structures disappeared in Tdo-/- mice. The cytoarchitectural organization of Tdo-/- mice was the same as that of wild type mice. Image collected and cropped by CiteAb from the following publication (http://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-3-26), licensed under a CC-BY licence.](http://images.novusbio.com/images2/TDO2-Antibody-Immunocytochemistry-Immunofluorescence-H00006999-B01P-img0009.jpg)
![Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal to the top). E: Horizontal section (immunofluorescent preparation, posterior to the top). Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Tyrosine-Hydroxylase-Antibody-Immunohistochemistry-NB300-109-img0029.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NB300-109-img0007.jpg)
![Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/Tyrosine-Hydroxylase-Antibody-Simple-Western-NB300-109-img0008.jpg)
![Western Blot: MAO-A Antibody [NBP2-38868] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/images2/MAO-A-Antibody-Western-Blot-NBP2-38868-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAO-A Antibody [NBP2-38868] - Staining of human cell line RT4 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/MAO-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38868-img0005.jpg)
![Immunohistochemistry-Paraffin: MAO-A Antibody [NBP2-38868] - Analysis in human small intestine and lymph node tissues using HPA059299 antibody. Corresponding MAOA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MAO-A-Antibody-Immunohistochemistry-Paraffin-NBP2-38868-img0010.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - (0.1ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/Phenylalanine Hydroxylase Antibody-Western Blot-NBP1-52084-img0004.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-52084-img0005.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Analysis of Phenylalanine Hydroxylase Human Liver lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/Phenylalanine Hydroxylase Antibody-Western Blot-NBP1-52084-img0002.jpg)
![Western Blot: COMT Antibody [NB100-41387] - Staining of human testis lysate (35ug protein in RIPA buffer) with Goat Anti-COMT (internal) (0.03ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/COMT-Antibody-Western-Blot-NB100-41387-img0003.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0006.jpg)
![Immunohistochemistry-Paraffin: COMT Antibody [NB100-41387] - (4ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/images2/COMT-Antibody-Immunohistochemistry-Paraffin-NB100-41387-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: 5-HT2A Antibody [NBP2-26091] - Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/images2/5-HT2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-26091-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-HT2A Antibody [NBP2-26091] - Staining of paraffin embedded Human Cerebellum. Antibody at 2 ug/mL. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/images2/5-HT2A-Antibody-Immunohistochemistry-Paraffin-NBP2-26091-img0002.jpg)
![Flow Cytometry: 5-HT2A Antibody [NBP2-26091] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/5-HT2A-Antibody-Flow-Cytometry-NBP2-26091-img0004.jpg)
![Western Blot: 5-HT1A Antibody [NBP2-21590] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT1A receptor antibody [N3C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/images2/5-HT1A-Antibody-Western-Blot-NBP2-21590-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: 5-HT1A Antibody [NBP2-21590] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/images2/5-HT1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21590-img0007.jpg)
![Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590] - Frozen Sectioned adult mouse hippocampus. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:250.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/images2/5-HT1A-Antibody-Immunohistochemistry-Frozen-NBP2-21590-img0008.jpg)
![Western Blot: Serotonin N-acetyltransferase Antibody [NB100-135] - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.](http://images.novusbio.com/images2/Serotonin N-acetyltransferase Antibody-Western Blot-NB100-135-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Serotonin N-acetyltransferase Antibody [NB100-135] - AANAT in COS7 and 1E7 cells detected by ICC. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.](http://images.novusbio.com/images2/Serotonin N-acetyltransferase Antibody-Immunocytochemistry-Immunofluorescence-NB100-135-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining in human adrenal gland and liver tissues using anti-QPCT antibody. Corresponding QPCT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Immunohistochemistry-Paraffin-NBP1-81838-img0010.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in control (vector only transfected HEK293T lysate) and QPCT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0011.jpg)
![Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.](http://images.novusbio.com/images2/Glutaminyl-peptide-Cyclotransferase-QPCT-Antibody-Western-Blot-NBP1-81838-img0006.jpg)
![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.](http://images.novusbio.com/images2/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-174-img0005.jpg)
![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.](http://images.novusbio.com/images2/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-174-img0004.jpg)
![Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/images2/Dopa-Decarboxylase-DDC-Antibody-Simple-Western-NB300-174-img0003.jpg)
![Immunohistochemistry-Paraffin: Dopamine Receptor D4 Antibody [NLS3921] - Anti-DRD4 / Dopamine Receptor D4 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/images2/Dopamine-Receptor-D4-Antibody-Immunohistochemistry-Paraffin-NLS3921-img0003.jpg)
![Immunohistochemistry-Paraffin: Dopamine Receptor D4 Antibody [NLS3921] - Staining of human brain, Neurons and Glia](http://images.novusbio.com/images2/Dopamine-Receptor-D4-Antibody-Immunohistochemistry-Paraffin-NLS3921-img0001.jpg)
![Immunohistochemistry-Paraffin: Dopamine Receptor D4 Antibody [NLS3921] - Analysis of anti-DRD4 / Dopamine Receptor D4 antibody with human brain, glioblastoma.](http://images.novusbio.com/images2/Dopamine-Receptor-D4-Antibody-Immunohistochemistry-Paraffin-NLS3921-img0002.jpg)
![Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.](http://images.novusbio.com/images2/5-HT1B-Antibody-Western-Blot-NB100-56350-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ASMT Antibody [NBP2-92663] - Analysis of MCF7 cells using ASMT.](http://images.novusbio.com/images2/ASMT Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92663-img0001.jpg)
