| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86922-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5276-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5276-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90022-50ul | |||
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Human melanoma cell line A375 and 5 patient melanoma tissue samples. 15 ug cell lysate per lane. TPH1 antibody at 1:400, 4C O/N. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NBP1-86922-img0020.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0019.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0011.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0013.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0015.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0018.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Western-Blot-NBP2-67580-img0004.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate")
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-Tryptophan Hydroxylase/TPH-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0005.jpg)
![Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Flow-Cytometry-NBP2-67580-img0001.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0003.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody at 1/1,000 dilution.Lane 1: MDA-MB-231-si NT cell lysateLane 2: MDA-MB-231-si TPH1 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 51 kDaObserved band size: 72 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-67580 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, (1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67580_rabbit-monoclonal-tryptophan-hydroxylase-1-tph-1-antibody-sc53-07-western-blot-462024131133..jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NB300-176-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NB300-176] - Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.")
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Western-Blot-NBP2-90022-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Tryptophan Hydroxylase 1/TPH-1 Antibody (145) [NBP2-90022] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate0.5 uL anti-TPH1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TPH1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 60 kDa](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-145-Immunoprecipitation-NBP2-90022-img0001.jpg)
![Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-[p-Ser58]-Antibody-Western-Blot-NB300-260-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.")
![Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-[p-Ser260]-Antibody-Western-Blot-NB300-307-img0001.jpg "Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.")
![ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Samples were spiked with high concentrations of Rat Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68167_rat-tryptophan-hydroxylase-1-tph-1-elisa-kit-colorimetric-132202416175614.jpg "ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Samples were spiked with high concentrations of Rat Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68167_rat-tryptophan-hydroxylase-1-tph-1-elisa-kit-colorimetric-4320249141637.jpg)
![ELISA: Human Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68157] - Samples were spiked with high concentrations of Human Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68157_human-tryptophan-hydroxylase-1-tph-1-elisa-kit-color-132202416211345.jpg "ELISA: Human Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68157] - Samples were spiked with high concentrations of Human Tryptophan Hydroxylase 1/TPH-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68157] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68157_human-tryptophan-hydroxylase-1-tph-1-elisa-kit-color-4320249173451.jpg)
![Western Blot: Tryptophan Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-17216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tryptophan Hydroxylase 1.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17216-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1 Overexpression Lysate (Adult Normal) [NBL1-17216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Tryptophan Hydroxylase 1.")