Tryptase gamma-1/TPSG1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

Tryptase gamma-1/TPSG1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Tryptase γ‑1/TPSG1
  Arg20-Arg281
  Accession # Q9NRR2
• Specificity: Detects human Tryptase gamma ‑1/TPSG1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptase gamma-1/TPSG1 Antibody [Alexa Fluor® 594]

• EC 3.4.21
• EC 3.4.21.-
• gamma I
• gamma II
• lung tryptase
• mast cell protease II
• mast cell tryptase
• PRSS31skin tryptase
• Serine protease 31
• TMTpituitary tryptase
• TPSG1
• Transmembrane tryptase
• trpA
• tryptase gamma 1
• tryptase gamma I
• tryptase gamma II
• tryptase gamma
• Tryptase gamma1
• Tryptase gamma-1

Background

As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including Tryptases, chymase and cathepsin G (4). Tryptase gamma -1, also called transmembrane Tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma -1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2). The rhTPSG1 was expressed as a soluble protein terminated at residue 281 and corresponded to the proenzyme. The proenzyme can be cleaved by trypsin to form the active enzyme. The serine protease activity of trypsin-activated rhTPSG1 can be inhibited by ecotin (R&D Systems, Catalog # 1328-PI). Greater than 95% protease activity is inhibited by ecotin at approximately 10:1 molar ratio.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.