TRPM2 Overexpression Lysate

Product Discontinued

TRPM2 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC222755 and based on accession number NM_020389. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens transient receptor potential cation channel, subfamily C, member 7 (TRPC7), mRNA.
• Gene: TRPM2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TRPM2 Overexpression Lysate

• EC 3.6.1.13
• EREG1MGC133383
• Estrogen-responsive element-associated gene 1 protein
• KNP3LTrpC-2
• Long transient receptor potential channel 2
• LTrpC2
• LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2
• NUDT9H
• NUDT9L1
• transient receptor potential cation channel, subfamily M, member 2
• Transient receptor potential channel 7
• TrpC7

Background

TRPM2 belongs to the ion transport protein family. This family contains Sodium, Potassium, Calcium and ion channels. This family has 6 transmembrane helices in which the last two helices flank a loop which determines ion selectivity. TRPM2 is activated by oxidative stress and confers susceptibility to cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TRPM2