TRPC6 Antibody

Western Blot: TRPC6 Antibody [NBP1-00142] - Analysis of TRPC6 in human placenta lysate (35ug protein in RIPA buffer) using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

TRPC6 Antibody Summary

• Immunogen: Peptide with sequence C-EKLSMEPNQEETNR corresponding to C-Terminus according to NP_004612.2.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRPC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human placenta lysates (calculated MW of 106Da according to NP_004612.2). IP usage is reported in scientific literature (PMID: 23948707).
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in the literature (PMID: 23657570).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPC6 Antibody

• FLJ11098
• FSGS2
• short transient receptor potential channel 6
• transient receptor potential cation channel, subfamily C, member 6
• Transient receptor protein 6
• TRP6
• TRP-6
• TRP6FLJ14863
• TRPC6

Background

The protein encoded by the TRPC6 gene creates receptor-activated cation permeant calcium channels in the cell membrane. Isoform 1 is 931 amino acids in length and is approximately 106 kDa, while isoform 2 is 815 amino acids in lenth at around 93 kDA. Isoform 3 is 876 amino acids long and is just over 100 kDA. Focal segmental glomerulosclerosis 2 (FSGS2) is caused by deficits in the TRPC6 gene. This gene has been linked to diseases such as cystic fibrosis, gigantism, hypertension, kidney disease, glomerulosclerosis, pyloric stenosis, diencephalic neoplasm, premature ovarian failure, and hepatopulmonary syndrome. The TRPC6 gene interacts with MX1, ORAI1, NPHS1, NPHS2, and FYN genes in pathways that monitor sodium as well as calcium channels, EPO signaling pathway, G alpha (q) signaling events, signal transduction, and platelet homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPC6 Antibody (NBP1-00142)(4)

Publications using NBP1-00142

• Alvarez-Miguel I, Cidad P, Perez-Garcia MT, Lopez-Lopez JR. Differences in TRPC3 and TRPC6 channels assembly in mesenteric vascular smooth muscle cells in essential hypertension. J. Physiol. (Lond.). 2016-11-12 [PMID: 27861908] (IP, Mouse)
• Kim EY, Anderson M, Wilson C et al. NOX2 interacts with podocyte TRPC6 channels and contributes to their activation by diacylglycerol: essential role of podocin in formation of this complex. Am J Physiol Cell Physiol. 2013-11-01 [PMID: 23948707] (IP, Mouse)
• Anderson M, Kim EY, Hagmann H et al. Opposing effects of podocin on the gating of podocyte TRPC6 channels evoked by membrane stretch or diacylglycerol. Am J Physiol Cell Physiol 2013-05-08 [PMID: 23657570] (WB, Mouse)
• Foller M, Kasinathan RS, Koka S et al. TRPC6 contributes to the Ca(2+) leak of human erythrocytes. Cell Physiol Biochem 2008-01-01 [PMID: 18209485]

Review for TRPC6 Antibody (NBP1-00142) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 02/09/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse mesenteric VSMCs
• Species: Mouse

Bioinformatics

• Gene Symbol: TRPC6
• Entrez:
  • 7225(Human)
• Uniprot:
  • Q9Y210(Human)