TROY/TNFRSF19 Antibody

Western Blot: TROY Antibody [NB120-12126] - Analysis of TROY using NB120-12126 in T98G, a human glioblastoma cell line lysate. A protein band of approximately 46 kD is detected.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TROY/TNFRSF19 Antibody Summary

• Description: Novus Biologicals Rabbit TROY/TNFRSF19 Antibody (NB120-12126) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This rabbit polyclonal antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 29-44, 204-219, and 409-421 of human TROY (Genbank Accession No. AB040434).
• Localization: Type I membrane protein (Probable).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFRSF19
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml
• Application Notes: WB: Detects a band of approximately 46 kDa (predicted molecular weight: 50.8 kDa). For optimal results, primary antibody incubations should be performed at room temperature.
• Control:

  TROY/TNFRSF19 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TROY/TNFRSF19 Antibody

• TAJ
• TAJ-alpha
• TAJToxicity and JNK inducer
• TNFRSF19
• TRADE
• TROY
• TROYtumor necrosis factor receptor superfamily member 19
• tumor necrosis factor receptor superfamily, member 19

Background

Analysis of EST clones and use of a signal sequence trap screening of the murine brain have lead to identification of a new member of the tumor necrosis factor receptor superfamily designated as TAJ and TROY, respectively. TAJ/TROY is a membrane protein of 423 amino acids with characteristic cysteine-rich motifs in the extracellular domain and a tumor necrosis factor receptor-associated factor (TRAF) 2 binding sequence in the cytoplasmic domain. Human TROY has 78% identity at the amino acid level with mouse TROY. The extracellular domain of TROY has significant homology with that of EDAR (another newly identified receptor belonging to TNF receptor family) that specifies hair follicle fate. The cytoplasmic domain of TAJ is different than other TNF receptor family members. Although its cytoplasmic domain does not contain death domain, TAJ/TROY induced cell death is probably mediated through a caspase-independent pathway. TAJ-induced JNK activation was not blocked by dominant-negative inhibitors of TRAF2, TRAF5, or ASK1, which have been previously implicated in JNK activation via TNFR1 and CD40. However, coimmunoprecipitation assays revealed that TAJ is capable of binding a number of different TRAF family members, and it is possible that TAJ-induced JNKactivation is mediated by an as yet untested TRAF homolog, such as TRAF6. TROY mRNA is highly expressed in brain, prostate and embryo and moderately expressed in the heart, lung, liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF19
• Entrez:
  • 55504(Human)
  • 29820(Mouse)
• Uniprot:
  • Q9NS68()