tropomyosin-4 Antibody - BSA Free

Western Blot: tropomyosin-4 Antibody [NBP2-15146] - Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: tropomyosin-4 Antibody [NBP2-15146] - Paraffin-embedded 4T1 xenograft, using antibody at 1:100 dilution.

Western Blot: tropomyosin-4 Antibody [NBP2-15146] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Western Blot: tropomyosin-4 Antibody [NBP2-15146] - Sample (30 ug of whole cell lysate) A: zebrafish heart B: zebrafish muscle 12% SDS PAGE NBP2-15146 diluted at 1:500

Immunohistochemistry: tropomyosin-4 Antibody [NBP2-15146] - tropomyosin-4 antibody detects tropomyosin-4 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. tropomyosin-4 antibody (NBP2-15146) dilution: 1:100.

• Reactivity: Hu, Mu, Rt, Ze, Po, Bv, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

tropomyosin-4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit tropomyosin-4 Antibody - BSA Free (NBP2-15146) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human tropomyosin-4. The exact sequence is proprietary.
• Predicted Species: Porcine (100%), Bovine (100%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPM4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24359494). Xenopus laevis (92%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for tropomyosin-4 Antibody - BSA Free

• TM30p1
• tropomyosin 4
• tropomyosin alpha-4 chain
• tropomyosin-4

Background

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TPM4
• Uniprot:
  • P67936(Human)