TROP-2 Antibody

Western Blot: TROP-2 Antibody [NBP1-05036] - (0.2ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP1-05036] - EB08839 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TROP-2 Antibody Summary

• Description: Novus Biologicals Goat TROP-2 Antibody (NBP1-05036) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TROP-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HYEQPTIQIELRQN corresponding to internal region according to NP_002344.2.
• Epitope: internal region
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TACSTD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.2-0.5 ug/ml
• Application Notes: Western blot: Approx. 37kDa band observed in Human Prostate lysates (calculated MW of 35.7kDa according to NP_002344.2). Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Tonsil.
• Control:

  TROP-2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TROP-2 Antibody

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (NBP1-05036)(1)

Publication using NBP1-05036

• Shimada A, Kano J, Ishiyama T et al. Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development. Cancer Sci 2005-10-01 [PMID: 16232198]

Bioinformatics

• Gene Symbol: TACSTD2
• Entrez:
  • 4070(Human)
  • 56753(Mouse)
• Uniprot:
  • NP_002344.2()