TROP-2 Antibody (MM0588-49D6) [Biotin]

Flow Cytometry: TROP-2 Antibody (MM0588-49D6) [Biotin] [NBP2-11982B] - An intracellular stain was performed on A431 cells with TROP-2 Antibody [MM0588-49D6] NBP2-11982B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: MM0588-49D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

TROP-2 Antibody (MM0588-49D6) [Biotin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Human recombinant TROP-2
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACSTD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TROP-2 Antibody (MM0588-49D6) [Biotin]

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (NBP2-11982B)(2)

Publications using NBP2-11982B

• Crovetti B, Maktabi MA, Erfani H et al. Circulating trophoblast numbers as a potential marker for pregnancy complications Prenatal Diagnosis 2022-08-01 [PMID: 35765264] (Human)
• Vossaert L, Wang Q, Salman R et al. Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis Am. J. Hum. Genet. 2019-11-26 [PMID: 31785788] (Human)
  
  Details:
  The biotin conjugated of the HLA G Antibody (G233), NB110-55298, was used for trophoblast enrichment.

Bioinformatics

• Gene Symbol: TACSTD2