TROP-2 Antibody (MM0588-49D6) - Azide and BSA Free

Flow Cytometry: TROP-2 Antibody (MM0588-49D6) [NBP2-11982] - An intracellular stain was performed on A431 cells with TROP-2 Antibody [MM0588-49D6] NBP2-11982B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).

Flow Cytometry: Mouse Monoclonal TROP-2 Antibody (MM0588-49D6) [NBP2-11982] - Detection of human TROP-2 on A431 epidermoid carcinoma cells. A431 cells were treated with the TROP-2 AF647 antibody (catalog # NBP2-11982AF647) (BLUE) or mouse IgG2 AF647 isotype control antibody (RED). Image from a verified customer review.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: MM0588-49D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TROP-2 Antibody (MM0588-49D6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TROP-2 Antibody (MM0588-49D6) - Azide and BSA Free (NBP2-11982) is a monoclonal antibody validated for use in WB and Flow. Anti-TROP-2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant TROP-2
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: TACSTD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for TROP-2 Antibody (MM0588-49D6) - Azide and BSA Free

• Cell surface glycoprotein Trop-2
• EGP1
• EGP-1
• epithelial glycoprotein-1
• GA733-1
• GA733-1GP50
• gastrointestinal tumor-associated antigen GA7331
• gp50
• M1S1cell surface glycoprotein TROP2
• Membrane component chromosome 1 surface marker 1
• membrane component, chromosome 1, surface marker 1
• Pancreatic carcinoma marker protein GA733-1
• pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
• T16
• TACSTD2
• TROP2
• TROP-2
• TROP2GA7331
• tumor-associated calcium signal transducer 2

Background

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TROP-2 Antibody (NBP2-11982)(2)

Publications using NBP2-11982

• Crovetti B, Maktabi MA, Erfani H et al. Circulating trophoblast numbers as a potential marker for pregnancy complications Prenatal Diagnosis 2022-08-01 [PMID: 35765264]
• Vossaert L, Wang Q, Salman R et al. Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis Am. J. Hum. Genet. 2019-11-26 [PMID: 31785788] (Human)
  
  Details:
  The biotin conjugated of the HLA G Antibody (G233), NB110-55298, was used for trophoblast enrichment.

Bioinformatics

• Gene Symbol: TACSTD2
• Uniprot:
  • P09758()