TRMT5 Antibody - BSA Free

Western Blot: TRMT5 Antibody [NBP1-82909] - Analysis in control (vector only transfected HEK293T lysate) and TRMT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: TRMT5 Antibody [NBP1-82909] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909] - Staining of human fallopian tube shows strong nuclear postivity in glandular cells.

Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909] - Staining of human prostate shows strong nuclear postivity in glandular cells.

Immunohistochemistry-Paraffin: TRMT5 Antibody [NBP1-82909] - Staining of human skeletal muscle shows no postivity in myocytes as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRMT5 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DGKDFLQGPVKEELMQLLGLSKERKPSVHVVMNLPAKAIEFLSAFKWLLDGQPCSSEFLPIVHCYSFSKDANPAEDVRQRAGAVLGISLEACSSVHLVRNVAPNKEMLCITFQIPASVLYKNQTRNPENHEDPPLKR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRMT5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICCIF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TRMT5 Protein (NBP1-82909PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TRMT5 Antibody - BSA Free

• KIAA1393EC 2.1.1.31
• M1G-methyltransferase
• TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae)
• TRM5MGC111453
• tRNA (guanine-N(1)-)-methyltransferase
• tRNA [GM37] methyltransferase
• tRNA methyltransferase 5
• tRNA-N1G37 methyltransferase

Background

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 (PubMed 15248782)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRMT5