TrkC Antibody (OTI2B8)

Western Blot: TrkC Antibody (2B8) [NBP1-47900] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TrkC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TrkC.

Immunocytochemistry/Immunofluorescence: TrkC Antibody (2B8) [NBP1-47900] Staining of COS7 cells transiently transfected by pCMV6-ENTRY TrkC.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TrkC mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TrkC Antibody (2B8) [NBP1-47900] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TrkC mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI2B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TrkC Antibody (OTI2B8) Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human NTRK3 expression vector
• Specificity: This antibody is specific for Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), transcript variant 3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NTRK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 94.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TrkC Antibody (OTI2B8)

• EC 2.7.10
• EC 2.7.10.1
• ETS related protein-neurotrophic receptor tyrosine kinase fusion protein
• ETV6-NTRK3 fusion
• gp145(trkC)
• GP145-TrkC
• Neurotrophic tyrosine kinase receptor type 3
• neurotrophic tyrosine kinase, receptor, type 3
• NT 3 receptor
• NT-3 growth factor receptor
• NTRK3
• TrkC tyrosine kinase
• TrkC
• trk-C
• TRKCneurotrophin 3 receptor
• tyrosine kinase receptor C

Background

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTRK3
• Entrez:
  • 4916(Human)
• Uniprot:
  • Q16288()