TrkA Antibody (165131R) [Allophycocyanin]

BAF cell line transfected with human TrkA and eGFP was stained with (A) Mouse Anti-Human TrkA APC-conjugated Monoclonal Antibody (Catalog # FAB1751RA) or (B) Mouse IgG1 isotype control antibody (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA APC-conjugated Monoclonal Antibody (Catalog # FAB1751RA, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

TrkA knockout K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA APC-connjugated Monoclonal Antibody (Catalog # FAB1751RA, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). No staining in the TrkA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, KO
• Clone: 165131R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

TrkA Antibody (165131R) [Allophycocyanin] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TrkA
  Ala33-Glu407
  Accession # P04629
• Specificity: Detects human TrkA-I and TrkA-II in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells
  • Knockout Validated

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkA Antibody (165131R) [Allophycocyanin]

• DKFZp781I14186
• EC 2.7.10
• EC 2.7.10.1
• MTChigh affinity nerve growth factor receptor
• Neurotrophic tyrosine kinase receptor type 1
• neurotrophic tyrosine kinase, receptor, type 1
• NTRK1
• NTRK-1
• p140-TrkA
• TRK1-transforming tyrosine kinase protein
• TrkA
• Trk-A
• TRKAOncogene TRK
• TRKTRK1
• tyrosine kinase receptor A

Background

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TrkA Antibody (FAB1751RA). (Showing 1 - 1 of 1 FAQ).

1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
   • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Bioinformatics

• Uniprot:
  • P04629()