TRIM37 Antibody - Azide and BSA Free

Western Blot: TRIM37 Antibody [NBP2-98831] - Anti-TRIM37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 128 kDa

Immunocytochemistry/Immunofluorescence: TRIM37 Antibody [NBP2-98831] - Immunofluorescence staining of TRIM37 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98831 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TRIM37 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TRIM37.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM37
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for TRIM37 Antibody - Azide and BSA Free

• EC 6.3.2.-
• KIAA0898Tripartite motif-containing protein 37
• Mulibrey nanism protein
• MULRING-B-box-coiled-coil protein
• POB1E3 ubiquitin-protein ligase TRIM37
• TEF3
• tripartite motif containing 37
• tripartite motif-containing 37

Background

TRIM37 encodes a member of the tripartite motif (TRIM) family, whose members are involved in diverse cellular functions such as developmental patterning and oncogenesis. The TRIM motif includes zinc-binding domains, a RING finger region, a B-box motif and a coiled-coil domain. The RING finger and B-box domains chelate zinc and might be involved in protein-protein and/or protein-nucleic acid interactions. The gene mutations are associated with mulibrey (muscle-liver-brain-eye) nanism, an autosomal recessive disorder that involves several tissues of mesodermal origin. Alternatively spliced transcript variants encoding the same protein have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM37