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![Western Blot: TRIM37 Antibody [NBP2-20715] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM37 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Western-Blot-NBP2-20715-img0004.jpg "Western Blot: TRIM37 Antibody [NBP2-20715] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM37 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Western Blot: TRIM37 Antibody [NBP2-20715] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/TRIM37 Antibody-Western Blot-NBP2-20715-img0001.jpg)
![Western Blot: TRIM37 Antibody [NBP2-20715] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM37 antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Western-Blot-NBP2-20715-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRIM37 Antibody [NBP2-80147] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80147-img0001.jpg "Immunocytochemistry/Immunofluorescence: TRIM37 Antibody [NBP2-80147] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: TRIM37 Antibody [NBP2-80147] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Flow-Cytometry-NBP2-80147-img0002.jpg)
![Western Blot: TRIM37 Antibody [NBP2-98831] - Anti-TRIM37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 128 kDa](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Western-Blot-NBP2-98831-img0002.jpg "Western Blot: TRIM37 Antibody [NBP2-98831] - Anti-TRIM37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 128 kDa")
![Immunocytochemistry/Immunofluorescence: TRIM37 Antibody [NBP2-98831] - Immunofluorescence staining of TRIM37 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti- TRIM37 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98831-img0001.jpg)
![Immunoprecipitation: TRIM37 Antibody [NB100-59820] - Detection of Human TRIM37 on HeLa whole cell lysate using NB100-59820. TRIM37 was also immunoprecipitated by rabbit anti-TRIM37 antibody NB100-59821. For blotting immunoprecipitated TRIM37 NB100-59821 was used at 1.0 ug/ml.](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Immunoprecipitation-NB100-59820-img0004.jpg "Immunoprecipitation: TRIM37 Antibody [NB100-59820] - Detection of Human TRIM37 on HeLa whole cell lysate using NB100-59820. TRIM37 was also immunoprecipitated by rabbit anti-TRIM37 antibody NB100-59821. For blotting immunoprecipitated TRIM37 NB100-59821 was used at 1.0 ug/ml.")
![Western Blot: TRIM37 Antibody [NB100-59821] - Detection of Human TRIM37 on HeLa whole cell lysate using NB100-59821. TRIM37 was also immunoprecipitated by rabbit anti-TRIM37 antibody NB100-59820.](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Western-Blot-NB100-59821-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TRIM37 Antibody [NB100-59821] - Detection of Human TRIM37 on HeLa whole cell lysate using NB100-59821. TRIM37 was also immunoprecipitated by rabbit anti-TRIM37 antibody NB100-59820.")
![Western Blot: TRIM37 Antibody [NBP1-92529] - Western blot analysis in human cell lines MCF-7 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Western-Blot-NBP1-92529-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: TRIM37 Antibody [NBP1-92529] - Western blot analysis in human cell lines MCF-7 and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![Western Blot: TRIM37 Antibody [NBP2-98830] - Anti-TRIM37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 108 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize2/TRIM37-Antibody-Western-Blot-NBP2-98830-img0001.jpg "Western Blot: TRIM37 Antibody [NBP2-98830] - Anti-TRIM37 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 108 kDa. Observed band size: 108 kDa (We are unsure as to the identity of these extra bands).")
![Sandwich ELISA: TRIM37 Antibody (2D11) [H00004591-M01] - Detection limit for recombinant GST tagged TRIM37 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/TRIM37 Antibody (2D11)-Sandwich ELISA-H00004591-M01-img0002.jpg "Sandwich ELISA: TRIM37 Antibody (2D11) [H00004591-M01] - Detection limit for recombinant GST tagged TRIM37 is approximately 0.3ng/ml as a capture antibody.")