TRIM2 Antibody

Images

 
Western Blot: TRIM2 Antibody [NB100-1218] - Staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TRIM2 Antibody Summary

Immunogen
Peptide with sequence DSGNHCFKVYRYLQ corresponding to C-Terminus according to NP_056086.2, NP_001123539.1.
Epitope
C-DSGNHCFKVYRYLQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Immunohistochemistry 6 - 12 ug/ml
  • Immunohistochemistry-Paraffin 6 - 12 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
Application Notes
IP successful usage on mouse protein is reported in scientific literature (PMID: 21478148). WB: Approx. 80+85 kDa band bands observed in rat brain lysates (calculated MW of 81.5 kDa band according to human NP_001123539.1, 84.4 according to human NP_056086.2 and 83.3 according to rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cortex) shows cytoplasm stainning in neuronal cell bodies.
Positive Control
TRIM2 Lysate (NBL1-17280)
Publications
Read Publications using
NB100-1218 in the following applications:

  • IP
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM2 Antibody

  • KIAA0517tripartite motif protein 2
  • RING finger protein 86
  • RNF86tripartite motif protein TRIM2
  • tripartite motif containing 2
  • tripartite motif-containing 2
  • tripartite motif-containing protein 2

Background

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00005706-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, RNAi
NBP2-53088
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-88792
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-82614
Species: Hu
Applications: IHC, IHC-P
NBP2-32482
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-56841
Species: Hu
Applications: WB, ICC/IF
NBP1-89150
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-82778
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2574
Species: Hu
Applications: CyTOF-ready, ICFlow
NBP1-91214
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-20310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37969
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-57502
Species: Hu
Applications: ICC/IF
NBP2-19474
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1218
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP, PEP-ELISA

Publications for TRIM2 Antibody (NB100-1218)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: IP.


Filter By Application
IP
(1)
All Applications
Filter By Species
Rat
(1)
All Species

Reviews for TRIM2 Antibody (NB100-1218) (0)

There are no reviews for TRIM2 Antibody (NB100-1218). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRIM2 Antibody (NB100-1218) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for TRIM2 Antibody (NB100-1218)

Discover related pathways, diseases and genes to TRIM2 Antibody (NB100-1218). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRIM2 Antibody (NB100-1218)

Discover more about diseases related to TRIM2 Antibody (NB100-1218).
 

Pathways for TRIM2 Antibody (NB100-1218)

View related products by pathway.

PTMs for TRIM2 Antibody (NB100-1218)

Learn more about PTMs related to TRIM2 Antibody (NB100-1218).

Blogs on TRIM2

There are no specific blogs for TRIM2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRIM2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TRIM2
Entrez
Uniprot