TRIM2 Antibody

Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry: TRIM2 Antibody [NB100-1218] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM2 Antibody Summary

• Immunogen: Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1.
• Epitope: C-DSGNHCFKVYRYLQ
• Specificity: This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 6 - 12 ug/ml
  • Immunohistochemistry-Paraffin 6 - 12 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1 ug/ml
• Application Notes: IP successful usage on mouse protein is reported in scientific literature (PMID: 21478148). WB: ~ 75-85kDa bands observed in rat brain lysates (calculated MW of 81.5 kDa band according to human NP_001123539.1, 84.4 according to human NP_056086.2 and 83.3 according to rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cortex) shows cytoplasm stainning in neuronal cell bodies. Use in ICC/IF reported in secitific publication PMID: 32698497
• Control:

  TRIM2 Overexpression Lysate

Reactivity Notes

Use in Mouse reported in secitific publication PMID: 32698497

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM2 Antibody

• KIAA0517tripartite motif protein 2
• RING finger protein 86
• RNF86tripartite motif protein TRIM2
• tripartite motif containing 2
• tripartite motif-containing 2
• tripartite motif-containing protein 2

Background

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM2 Antibody (NB100-1218)(3)

Publications using NB100-1218

• Lokapally A, Neuhaus H, Herfurth J, Hollemann T Interplay of TRIM2 E3 Ubiquitin Ligase and ALIX/ESCRT Complex: Control of Developmental Plasticity During Early Neurogenesis Cells 2020-07-20 [PMID: 32698497] (ICC/IF, Mouse)
• Thompson S, Pearson A, Ashley M et al. Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection J Biol Chem 2011-06-03 [PMID: 21478148] (IP, Rat)
• Reymond A, Meroni G, Fantozzi A et al. The tripartite motif family identifies cell compartments. EMBO J 2001-05-01 [PMID: 11331580]

Bioinformatics

• Gene Symbol: TRIM2
• Entrez:
  • 23321(Human)
  • 80890(Mouse)
• Uniprot:
  • NP_001123539.1(Human)
  • NP_001337983.1(Human)
  • NP_001337984.1(Human)
  • NP_001337985.1(Human)
  • NP_001337986.1(Human)
  • NP_056086.2(Human)