TRIM2 Antibody

Images

 
Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: TRIM2 Antibody [NB100-1218] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TRIM2 Antibody Summary

Immunogen
Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1.
Epitope
C-DSGNHCFKVYRYLQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 6 - 12 ug/ml
  • Immunohistochemistry-Paraffin 6 - 12 ug/ml
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1 ug/ml
Application Notes
IP successful usage on mouse protein is reported in scientific literature (PMID: 21478148). WB: ~ 75-85kDa bands observed in rat brain lysates (calculated MW of 81.5 kDa band according to human NP_001123539.1, 84.4 according to human NP_056086.2 and 83.3 according to rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cortex) shows cytoplasm stainning in neuronal cell bodies. Use in ICC/IF reported in secitific publication PMID: 32698497
Control
TRIM2 Overexpression Lysate
Publications
Read Publications using
NB100-1218 in the following applications:

Reactivity Notes

Use in Mouse reported in secitific publication PMID: 32698497

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM2 Antibody

  • KIAA0517tripartite motif protein 2
  • RING finger protein 86
  • RNF86tripartite motif protein TRIM2
  • tripartite motif containing 2
  • tripartite motif-containing 2
  • tripartite motif-containing protein 2

Background

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIM2 Antibody (NB100-1218)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM2
Entrez
Uniprot