TRIB1 Antibody

Western Blot: TRIB1 Antibody [NBP2-27557] - (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIB1 Antibody Summary

• Description: Novus Biologicals Goat TRIB1 Antibody (NBP2-27557) is a polyclonal antibody validated for use in WB and ELISA. Anti-TRIB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence PEHVSPKARCLIRS corresponding to internal region according to NP_653132.1.
• Predicted Species: Human (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 42 kDa band observed in NIH3T3 lysates (calculated MW of 41.3 kDa band according to NP_653132.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIB1 Antibody

• C8FWG-protein-coupled receptor induced protein
• GIG-2
• GIG2G-protein-coupled receptor-induced protein 2
• G-protein-coupled receptor-induced gene 2 protein
• SKIP1
• TRB-1
• TRB1phosphoprotein regulated by mitogenic pathways
• tribbles homolog 1 (Drosophila)
• tribbles homolog 1
• tribbles-like protein 1

Background

Interacts with MAPK kinases and regulates activation of MAP kinases. May not display kinase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIB1 Antibody (NBP2-27557)(1)

Publication using NBP2-27557

• Yokoyama T, Kanno Y, Yamazaki Y et al. Trib1 links the MEK1/ERK pathway in myeloid leukemogenesis. Blood 2010-10-01 [PMID: 20610816]

Bioinformatics

• Gene Symbol: TRIB1
• Entrez:
  • 211770(Mouse)
  • 78969(Rat)
• Uniprot:
  • NP_653132.1(Human)