TRF-2 Antibody

Images

 
Immunoprecipitation: TRF-2 Antibody [NB100-56694] - Analysis of TRF2 in HL60 cells. Lane 1. IP (mouse anti-TRF2). Lane 2. IP with control mouse IgG. Lane 3 IP with goat anti-TRF2. Lane 4. IP with pre-immune goat Ig. ...read more
Immunohistochemistry-Paraffin: TRF-2 Antibody [NB100-56694] - Analysis of Human Tonsil using TRF2 antibody at 15 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TRF-2 Antibody Summary

Immunogen
Baculovirus expressed His-tagged whole length TRF2 protein was used for immunizing goat (NP_005643).
Marker
Telomeres marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TERF2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 15 ug/ml
  • Immunoprecipitation 2 ug/ 10^6 cells
Positive Control
TRF-2 Lysate (NBL1-16815)
Publications
Read Publications using
NB100-56694 in the following applications:

  • 2 publications
  • 2 publications
  • IF
    1 publication
  • IP
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRF-2 Antibody

  • Telomeric DNA-binding protein
  • telomeric repeat binding factor 2
  • Telomeric repeat binding protein 2
  • telomeric repeat-binding factor 2
  • TERF 2
  • TERF2
  • TRBF 2
  • TRBF2
  • TRF 2
  • TRF2
  • TRF-2
  • TTAGGG repeat binding factor 2
  • TTAGGG repeat-binding factor 2

Background

TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56694
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
Supplier Logo

Publications for TRF-2 Antibody (NB100-56694)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ChIP, ICC/IF, IF, IP, WB.


Filter By Application
ChIP
(2)
ICC/IF
(2)
IF
(1)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-56694 Applications Species
Charif R, Granotier-Beckers C, Bertrand HC et al. Association of a Platinum Complex to a G-Quadruplex Ligand Enhances Telomere Disruption Chem. Res. Toxicol. 2017 Jul 18 [PMID: 28657713] (IP) IP
Maida Y, Yasukawa M, Okamoto N et al. Involvement of TERT in heterochromatin maintenance. Mol. Cell. Biol. 2014 Feb 18 [PMID: 24550003] (ICC/IF, Human)

Details:
HeLa, Fig 1E
ICC/IF Human
Klein O, Rohwer N, de Molina KF et al. Application of two-dimensional gel-based mass spectrometry to functionally dissect resistance to targeted cancer therapy. Proteomics Clin Appl 2013 Dec [PMID: 24307263] (WB, Human) WB Human
Ourliac-Garnier Isabelle, Poulet Anais, Charif Razan et al. Platination of telomeric DNA by cisplatin disrupts recognition by TRF2 and TRF1. J Biol Inorg Chem. 2010 Jun [PMID: 20191372]
Caslini C, Connelly JA, Serna A et al. MLL associates with telomeres and regulates telomeric repeat-containing RNA transcription. Mol Cell Biol. 2009 Aug [PMID: 19528237] (WB, ChIP, IF)

Details:
Antibodies cited: TRF2 mAb (IMG-124a) and TRF2 pAb (IMG-148a) for ChIP, IF and WB. Cell llnes utilized: acute lymphoblastic leukemia (ALL) RS4;11 and ALL-PO cell lines, and the monoblastic, pre-B, and T-lineage leukemia cell lines U937, Nalm6, and Jurkat,
WB, ChIP, IF
Raz V, Vermolen BJ, Garini Y et al. The nuclear lamina promotes telomere aggregation and centromere peripheral localization during senescence of human mesenchymal stem cells. J Cell Sci. 2008 Dec 15 [PMID: 19056671] (ICC/IF, Human)

Details:
IF (human mesenchymal stem cells), Fig. 5A.
ICC/IF Human
Razak ZR, Varkonyi RJ, Kulp-McEliece M et al. p53 differentially inhibits cell growth depending on the mechanism of telomere maintenance. Mol Cell Biol. 2004 Jul [PMID: 15199150] (ChIP) ChIP
Mearini G, Chichiarelli S, Zampieri M et al. Interaction of EBV latent origin of replication with the nuclear matrix: identification of S/MAR sequences and protein components. FEBS Lett. 2003 Jul 17 [PMID: 12860398] (WB, Human)

Details:
TRF-2 (IMG-148A). WB: EBV-positive and EBV-negative human lymphoma cells, Fig 5.
WB Human

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for TRF-2 Antibody (NB100-56694). (Showing 1 - 4 of 4 FAQs).

  1. Are the TRF-2 antibodies validated in Simple Western?
    • Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.
  2. Does TRF-2 antibodies comes in lyophilized form?
    • we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.
  3. What the theoretical molecular weight for TRF-2 antibodies?
    • The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
  4. What is the immunogen sequence of this TRF-2 antibody?
    • The whole length of Baculovirus expressed His-tagged TRF-2 protein is proprietary.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TRF-2 Antibody (NB100-56694)

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Blogs on TRF-2.

Application Highlight: Recent uses of TERF2 in immunofluorescence (IF)
Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in th...  Read full blog post.

TRF2: Telomere Maintenance and Protection
TRF2 (telomeric repeat-binding factor 2) is a homodimeric protein that binds the double-stranded 5'-TTAGGG-3' repeat in telomeres. It plays a critical role in the maintenance of telomeres, protecting telomeres against end-to-end chromosomal fusion, an...  Read full blog post.

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Bioinformatics

Gene Symbol TERF2
Entrez
Uniprot