Transketolase Antibody

Immunocytochemistry/Immunofluorescence: Transketolase Antibody [NBP2-20699] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Western Blot: Transketolase Antibody [NBP2-20699] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:1000.

Transketolase antibody [N3C2], Internal detects Transketolase protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Transketolase antibody [N3C2], Internal (NBP2-20699) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (NBP2-20699) diluted at 1:1000.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transketolase antibody [N3C2], Internal (NBP2-20699) diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Transketolase Antibody Summary

• Description: Novus Biologicals Rabbit Transketolase Antibody (NBP2-20699) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Transketolase. The exact sequence is proprietary.
• Predicted Species: Rat (98%), Porcine (99%), Bovine (97%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TKT
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Transketolase Antibody

• EC 2.2.1.1
• FLJ34765
• TK
• TKT1
• transketolase

Background

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TKT
• Uniprot:
  • P29401(Human)