Transglutaminase 5 Antibody - BSA Free

Western Blot: Transglutaminase 5 Antibody - BSA Free [Transglutaminase 5] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Transglutaminase 5 using Transglutaminase 5 Rabbit pAb at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.

Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded rat kidney using Transglutaminase 5 .

Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded human colon using Transglutaminase 5 .

Immunohistochemistry-Paraffin: Transglutaminase 5 Antibody [NBP2-94524] - Paraffin-embedded mouse heart using Transglutaminase 5 .

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Transglutaminase 5 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2). LQKLKARSFHGSQRGAELQPSRPTSLSQDSPRSLHTPSLRPSDVVQVSLKFKLLDPPNMGQDICFVLLALNMSSQFKDLKVNLSAQSLLHDGSPLSPFWQDTAFITLSPKEAKTYPCKISYSQYSQYLSTDKLIRISALGEEKSSPEKILVNKIITLSYPSITINVLGAAVVNQPLSIQVIFSNPLSEQVEDCVLTVEGSGLFKKQQKVFLGVLKPQHQASIILETVPFKSGQRQIQANMRSNKFKDIKGYRNVYVDFAL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGM5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000 - 1:8000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Transglutaminase 5 Antibody - BSA Free

• EC 2.3.2.13
• protein-glutamine gamma-glutamyltransferase 5
• PSS2
• TG(X)
• TGase X
• TGASE5
• TGase-5
• TGASEX
• TGM5
• TGM6
• TGMX
• TGXMGC141907
• Transglutaminase 5
• transglutaminase V
• Transglutaminase X
• transglutaminase-5

Background

Tissue transglutaminase is an enzyme which catalyzes the cross linking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium dependent. Transglutaminase 5 contributes to the formation of the cornified cell envelope of keratinocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 5 Antibody (NBP2-94524)(1)

Publication using NBP2-94524

• Lee WS, Al-Ramahi I, Jeong HH et al. Cross-species genetic screens identify transglutaminase 5 as a regulator of polyglutamine-expanded ataxin-1 The Journal of clinical investigation May 2 2022 [PMID: 35499073] (WB, IHC-P, Mouse, Human)

Bioinformatics

• Gene Symbol: TGM5