Transglutaminase 2/TGM2 Antibody

Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Transglutaminase 2/TGM2 Polyclonal Antibody (Catalog # AF5418) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 2/TGM2 at approximately 80-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Transglutaminase 2/TGM2 Antibody Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Transglutaminase 2/TGM2
  Ala2-Ala686
  Accession # P21981
• Specificity: Detects mouse and rat Transglutaminase 2/TGM2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TGM2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Transglutaminase 2/TGM2 Antibody

• C polypeptide
• EC 2.3.2.13
• G-ALPHA-h
• protein-glutamine gamma-glutamyltransferase 2
• protein-glutamine-gamma-glutamyltransferase
• TG(C)
• TG2
• TGase C
• TGase H
• TGase-2
• TGase-H
• TGC
• TGCGNAH
• TGM2
• Tissue transglutaminase
• transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Transglutaminase 2
• Transglutaminase C
• Transglutaminase H
• transglutaminase-2
• tTG

Background

Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).

1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
2. Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
3. Griffin, M. et al. (2002) Biochem. J. 368:377.
4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGM2
• Uniprot:
  • P21981()