Transglutaminase 2/TGM2 Antibody (SPM358)

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (SPM358) [NBP2-44595] - Formalin-fixed, paraffin-embedded human Placenta stained with Transglutaminase 2/TGM2 Antibody (SPM592).

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, Flow, IHC
• Clone: SPM358
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Transglutaminase 2/TGM2 Antibody (SPM358) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47927)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human Transglutaminase 2/TGM2 protein (Uniprot: P21980)
• Localization: Cytoplasmic and cell surface
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TGM2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Transglutaminase 2/TGM2 Antibody (SPM358)

• C polypeptide
• EC 2.3.2.13
• G-ALPHA-h
• protein-glutamine gamma-glutamyltransferase 2
• protein-glutamine-gamma-glutamyltransferase
• TG(C)
• TG2
• TGase C
• TGase H
• TGase-2
• TGase-H
• TGC
• TGCGNAH
• TGM2
• Tissue transglutaminase
• transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Transglutaminase 2
• Transglutaminase C
• Transglutaminase H
• transglutaminase-2
• tTG

Background

TGase II is implicated in programmed cell death, signal transduction, drugresistance, cell growth, endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 2/TGM2 Antibody (NBP2-44595)(2)

Publications using NBP2-44595

• Boxhammer E, Lehle K, Schmid C, Von Suesskind-Schwendi M Anti-oxidative effect of the tyrosine kinase inhibitor nintedanib: a potential therapy for chronic lung allograft dysfunction? Exp. Lung Res. 2020-03-13 [PMID: 32169022] (IF/IHC, Rat)
• Markel Justin E, Noore Jabeen, Emery Eric J et al Using the Spleen as an In Vivo Systemic Immune Barometer Alongside Osteosarcoma Disease Progression and Immunotherapy with alpha-PD-L1. Sarcoma. 2018-12-12 [PMID: 30651716] (FLOW)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.

Bioinformatics

• Gene Symbol: TGM2
• Uniprot:
  • P21980()
  • P21980()
  • P21980()