Transferrin Antibody (HTF-14)

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Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ...read more

Product Details

Summary
Reactivity Hu, Po, RbSpecies Glossary
Applications WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
Clone
HTF-14
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Transferrin Antibody (HTF-14) Summary

Immunogen
Purified porcine transferrin.
Localization
Secreted
Specificity
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TF
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Blocking/Neutralizing 50-200 molar excess
  • ELISA 1:2000RadioImmunoassay 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Radioimmunoassay 1:100-1:2000
Application Notes
Western Blot: use non-reducing conditions. The antibody HTF-14 blocks binding of transferrin to the receptor. Purity is > 95% (by SDS-PAGE).
Positive Control
Transferrin Lysate (NBL1-16827)
Reviewed Applications
Read 1 Review rated 5
using
NB500-418 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Transferrin Antibody (HTF-14)

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA

Publications for Transferrin Antibody (NB500-418) (0)

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Review for Transferrin Antibody (NB500-418) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 application: WB.

Reviews using NB500-418:
Filter by Applications
WB
(1)
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reviewed by:
Alissa Taylor
WB 09/24/2014
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Summary

ApplicationWestern Blot

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Transferrin Antibody (NB500-418) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-418AF405
Alexa Fluor 488 Labeled NB500-418AF488
Alexa Fluor 647 Labeled NB500-418AF647
Alexa Fluor 700 Labeled NB500-418AF700
DyLight 350 Labeled NB500-418UV
DyLight 405 Labeled NB500-418V
DyLight 488 Labeled NB500-418G
DyLight 680 Labeled NB500-418FR
DyLight 755 Labeled NB500-418IR

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Bioinformatics Tool for Transferrin Antibody (NB500-418)

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Recent Reviews

5
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1
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1
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Alissa Taylor
09/24/2014
Application: WB
Species:

Bioinformatics

Gene Symbol TF
Entrez