Transferrin Antibody (HTF-14) - BSA Free

Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)

Immunohistochemistry-Paraffin: Transferrin Antibody (HTF-14) [NB500-418] - Staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).

• Reactivity: Hu, Po, Rb, Bv(-), Ca(-), Eq(-), Sh(-)
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, RIA, B/N
• Clone: HTF-14
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Transferrin Antibody (HTF-14) - BSA Free Summary

• Immunogen: Purified porcine transferrin.
• Localization: Secreted
• Specificity: The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:2000RadioImmunoassay 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 1-2 ug/ml
• Application Notes: Western Blot: use non-reducing conditions. The antibody HTF-14 blocks binding of transferrin to the receptor. Purity is > 95% (by SDS-PAGE).
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Transferrin Antibody (HTF-14) - BSA Free

• Beta-1 metal-binding globulin
• DKFZp781D0156
• EC 3.4.21
• PRO1557
• PRO2086
• Serotransferrin
• Siderophilin
• TF
• Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Transferrin Antibody (NB500-418) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer WB 09/24/2014

Summary

• Application: Western Blot

Bioinformatics

• Gene Symbol: TF
• Entrez:
  • 7018(Human)