Transferrin Antibody (HTF-14) - BSA Free

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Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ...read more
Immunohistochemistry-Paraffin: Transferrin Antibody (HTF-14) [NB500-418] - Staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).

Product Details

Summary
Reactivity Hu, Po, Rb, Bv(-), Ca(-), Eq(-), Sh(-)Species Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, B/N
Clone
HTF-14
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Transferrin Antibody (HTF-14) - BSA Free Summary

Immunogen
Purified porcine transferrin.
Localization
Secreted
Specificity
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TF
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA 1:2000RadioImmunoassay 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 1-2 ug/ml
Application Notes
Western Blot: use non-reducing conditions. The antibody HTF-14 blocks binding of transferrin to the receptor. Purity is > 95% (by SDS-PAGE).
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB500-418 in the following applications:

Publications
Read Publication using
NB500-418 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Transferrin Antibody (HTF-14) - BSA Free

  • Beta-1 metal-binding globulin
  • DKFZp781D0156
  • EC 3.4.21
  • PRO1557
  • PRO2086
  • Serotransferrin
  • Siderophilin
  • TF
  • Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transferrin Antibody (NB500-418)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Transferrin Antibody (NB500-418) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NB500-418:
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WB
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Alissa Taylor
WB 09/24/2014
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Summary

ApplicationWestern Blot

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Transferrin Antibody (NB500-418) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Transferrin Antibody (NB500-418)

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Blogs on Transferrin.

Transferrin: "Ironing" out the Details of Cellular Anemia
Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding...  Read full blog post.

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Recent Reviews

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1
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Alissa Taylor
09/24/2014
Application: WB
Species:

Bioinformatics

Gene Symbol TF
Entrez