TRANCE/TNFSF11/RANK L Antibody [Unconjugated]

In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 µg/mL.

MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0096286), licensed under a CC-BY license. Not internally tested by R&D Systems.

Tnfaip3 I325N mice exhibit impaired mammary development, elevated non-canonical RankL signalling and dampened non-canonical NF-kappa B signalling. (A) Mammary gland whole mounts of the 3rd and 4th mammary gland from 14-week virgin Tnfaip3I325N mice of indicated genotype. * denotes empty fat pad space. LN = lymph node. (B) Representative H&E of mammary gland from does 8.5 days post-partum, and (C) pup weight of resulting pairs, normalised to litter size. (D, E) Quantified positive immunohistochemistry staining for (D) progesterone receptor (PR) or (E) estrogen receptor (ER) (n = 9 Tnfaip3+/+ and 9 Tnfaip3I325N/I325N). (F) 4th Mammary gland from 10-week-old mice were collected and lysed for immunoblot assessment of paracrine hormones OPG and RANKL with Beta-actin used as the loading control.* denotes non-specific band and arrow head denotes specific band. Molecular weight in Kilodaltons is shown to the right of each blot. (G–I) 4th Mammary gland and its lymph node (J–L) from 10-week-old mice were collected for immunoblot (G, J) and densitometry analysis (H, I, K, L) for non-canonical NF-kappa B components NIK, p100/p52 and RelB. Immunoblots are representative of 2 independent experiments with n=6 (H, I) or n = 3 (K, L) biological replicates for both WT and HOM donors. Densitometry values were normalised to the average WT value of each blot to allow cumulative quantification. Error bars represent s.e.m and Student’s T test used for significance analysis * = P < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35464428), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC, B/N, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TRANCE/TNFSF11/RANK L Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L
  Arg72-Asp316
  Accession # O35235
• Specificity: Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF11
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.02-0.06 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse TRANCE/TNFSF11/RANK L Antibody (Catalog # AF462)
  ELISA Detection: Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462)
  Standard: Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody [Unconjugated]

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
2. Anderson, D.M. et al. (1997) Nature 390:175.
3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
4. Kong, Y-Y. et al. (1999) Nature 397:315.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (AF462)(35)

Publications using AF462

• Woo, HE;Cho, JY;Lim, YH; Propionibacterium freudenreichii MJ2-derived extracellular vesicles inhibit RANKL-induced osteoclastogenesis and improve collagen-induced rheumatoid arthritis Scientific reports 2024-10-23 [PMID: 39443658] (Immunocytochemistry, Mouse)
• Northey, JJ;Hayward, MK;Yui, Y;Stashko, C;Kai, F;Mouw, JK;Thakar, D;Lakins, JN;Ironside, AJ;Samson, S;Mukhtar, RA;Hwang, ES;Weaver, VM; Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer risk Cell stem cell 2024-01-04 [PMID: 38181747] (Immunohistochemistry, Mouse)
• Yamamoto, T;Abe, M;Hongo, H;Maruoka, H;Yoshino, H;Haraguchi-Kitakamae, M;Udagawa, N;Li, M;Amizuka, N;Hasegawa, T; Differential osteoblastic activity in primary metaphyseal trabecular and secondary trabeculae of c-fos deficient mice Journal of oral biosciences 2023-08-16 [PMID: 37595744] (IHC, Mouse)
• ZZ Lai, WJ Zhou, JW Shi, YH Meng, JN Wu, JF Ye, T Peng, CE Xu, MQ Li RANKL up-regulated by progesterone aggravates lipopolysaccharide-induced acute lung injury during pregnancy Journal of reproductive immunology, 2022-12-16;155(0):103788. 2022-12-16 [PMID: 36580846] (Neutralization, Mouse)
• Alfredo Cappariello, Maurizio Muraca, Anna Teti, Nadia Rucci Circulating Extracellular Vesicles Express Receptor Activator of Nuclear Factor kappa B Ligand and Other Molecules Informative of the Bone Metabolic Status of Mouse Models of Experimentally Induced Osteoporosis Calcified Tissue International 10/25/2022 [PMID: 36282293]
• S Sonoda, S Murata, H Yamaza, R Yuniartha, J Fujiyoshi, K Yoshimaru, T Matsuura, Y Oda, S Ohga, T Tajiri, T Taguchi, T Yamaza Targeting hepatic oxidative stress rescues bone loss in liver fibrosis Molecular Metabolism, 2022-09-13;66(0):101599. 2022-09-13 [PMID: 36113772] (Neutralization, Mouse)
• MM Szwarc, L Hai, VK Maurya, K Rajapakshe, D Perera, MM Ittmann, Q Mo, Y Lin, ML Bettini, C Coarfa, JP Lydon Histopathologic and transcriptomic phenotypes of a conditional RANKL transgenic mouse thymus Cytokine, 2022-09-11;160(0):156022. 2022-09-11 [PMID: 36099756] (IHC, Western Blot, Transgenic Mouse)
• Robert M. Gutgesell, Laiba Jamshed, Richard A. Frank, L. Mark Hewitt, Philippe J. Thomas, Alison C. Holloway Naphthenic acid fraction components from oil sands process‐affected water from the Athabasca Oil Sands Region impair murine osteoblast differentiation and function Journal of Applied Toxicology 2022-12-01 [PMID: 35894097] (Western Blot, Mouse)
• Robert M. Gutgesell, Laiba Jamshed, Richard A. Frank, L. Mark Hewitt, Philippe J. Thomas, Alison C. Holloway Naphthenic acid fraction components from oil sands process‐affected water from the Athabasca Oil Sands Region impair murine osteoblast differentiation and function Journal of Applied Toxicology 12/1/2022 [PMID: 35894097]
• SH Lee, YHY Yap, CL Lim, ARE Woo, VCL Lin Activation function 1 of progesterone receptor is required for mammary development and regulation of RANKL during pregnancy Scientific Reports, 2022-07-19;12(1):12286. 2022-07-19 [PMID: 35854046] (IHC, Mouse)
• M Inoue, Y Nagai-Yosh, R Yamasaki, T Kawamoto, T Nishihara, W Ariyoshi Mechanisms involved in suppression of osteoclast supportive activity by transforming growth factor-beta1 via the ubiquitin-proteasome system PLoS ONE, 2022-02-23;17(2):e0262612. 2022-02-23 [PMID: 35196318] (Western Blot, Mouse)
• Jingke Du, Zihao He, Junqi Cui, Hanjun Li, Mingming Xu, Shuhong Zhang et al. Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss Frontiers in Bioengineering and Biotechnology 11/11/2021 [PMID: 34858954]
• D Li, M San, J Zhang, A Yang, W Xie, Y Chen, X Lu, Y Zhang, M Zhao, X Feng, Y Zheng Oxytocin receptor induces mammary tumorigenesis through prolactin/p-STAT5 pathway Cell Death & Disease, 2021-06-07;12(6):588. 2021-06-07 [PMID: 34099636] (Western Blot, Mouse)
• HN Kim, J Xiong, RS MacLeod, S Iyer, Y Fujiwara, KM Cawley, L Han, Y He, JD Thostenson, E Ferreira, RL Jilka, D Zhou, M Almeida, CA O'Brien Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence JCI Insight, 2020-10-02;0(0):. 2020-10-02 [PMID: 32870816] (Western Blot, Mouse)
• M Li, D Xu Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site Proc. Natl. Acad. Sci. U.S.A., 2020-07-07;0(0):. 2020-07-07 [PMID: 32636266] (ICC, Proximity Ligation Assay, Mouse)
• R Pascual, J Martín, F Salvador, O Reina, V Chanes, A Millanes-R, C Suñer, G Fernández-, A Bartomeu, YS Huang, RR Gomis, R Méndez The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer Sci Adv, 2020-05-15;6(20):eaax3868. 2020-05-15 [PMID: 32440535] (IHC, Human)
• L Hai, MM Szwarc, DM Lonard, K Rajapakshe, D Perera, C Coarfa, M Ittmann, R Fernandez-, JP Lydon Short-term RANKL exposure initiates a neoplastic transcriptional program in the basal epithelium of the murine salivary gland Cytokine, 2019-06-18;123(0):154745. 2019-06-18 [PMID: 31226438] (IHC, Human)
• J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis J Transl Med, 2019-03-15;17(1):84. 2019-03-15 [PMID: 30876479] (IHC-P, Mouse)
• Dan Li, Yan Ji, Chunlan Zhao, Yapeng Yao, Anlan Yang, Honghong Jin et al. OXTR overexpression leads to abnormal mammary gland development in mice Journal of Endocrinology 11/1/2018 [PMID: 30089682]
• J Xiong, K Cawley, M Piemontese, Y Fujiwara, H Zhao, JJ Goellner, CA O'Brien Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss Nat Commun, 2018-07-25;9(1):2909. 2018-07-25 [PMID: 30046091] (Western Blot, Mouse)
• Lauren B. Rodda, Erick Lu, Mariko L. Bennett, Caroline L. Sokol, Xiaoming Wang, Sanjiv A. Luther et al. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity Immunity 5/1/2018 [PMID: 29752062]
• X Zhang, HC Chiang, Y Wang, C Zhang, S Smith, X Zhao, SJ Nair, J Michalek, I Jatoi, M Lautner, B Oliver, H Wang, A Petit, T Soler, J Brunet, F Mateo, M Angel Puja, E Poggi, K Chaldekas, C Isaacs, BN Peshkin, O Ochoa, F Chedin, C Theoharis, LZ Sun, TJ Curiel, R Elledge, VX Jin, Y Hu, R Li Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis Nat Commun, 2017-06-26;8(0):15908. 2017-06-26 [PMID: 28649985] (IHC-P, Mouse)
• Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts. J Biol Chem, 2015-06-17;290(33):20147-58. 2015-06-17 [PMID: 26085099] (Neutralization, Mouse)
• Maria M. Szwarc, Ramakrishna Kommagani, Allison P. Jacob, William C. Dougall, Michael M. Ittmann, John P. Lydon Aberrant Activation of the RANK Signaling Receptor Induces Murine Salivary Gland Tumors PLOS ONE 6/10/2015 [PMID: 26061636]
• Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity. Autophagy, 2014-10-30;10(11):2036-52. 2014-10-30 [PMID: 25483966] (IHC, Mouse)
• Wang L, Foster B, Kram V, Nociti F, Zerfas P, Tran A, Young M, Somerman M Fibromodulin and Biglycan Modulate Periodontium through TGFbeta/BMP Signaling. J Dent Res, 2014-06-25;93(8):780-787. 2014-06-25 [PMID: 24966230] (IHC-P, Mouse)
• Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling. PLoS ONE, 2014-05-05;9(5):e96286. 2014-05-05 [PMID: 24798452] (IHC-P, Mouse)
• Bruno R, Boulanger C, Rosenfield S, Anderson L, Lydon J, Smith G Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells. J Cell Sci, 2013-11-04;127(0):27-32. 2013-11-04 [PMID: 24190884] (IHC-P, Mouse)
• Sundaram K, Rao D, Ries W, Reddy S CXCL5 stimulation of RANK ligand expression in Paget&#039;s disease of bone. Lab Invest, 2013-02-25;93(4):472-9. 2013-02-25 [PMID: 23439434] (Western Blot, Human)
• Jaya Nautiyal, Jennifer H. Steel, Meritxell Rosell Mane, Olayiwola Oduwole, Ariel Poliandri, Xanthippi Alexi et al. The transcriptional co-factor RIP140 regulates mammary gland development by promoting the generation of key mitogenic signals Development 3/1/2013 [PMID: 23404106]
• Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer. Nature, 2010-09-29;468(7320):98-102. 2010-09-29 [PMID: 20881962] (Western Blot, Mouse)
• Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse. Dev. Biol., 2009-01-23;328(1):127-39. 2009-01-23 [PMID: 19298785] (Western Blot, Mouse)
• Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice. Circulation, 2008-01-02;117(3):411-20. 2008-01-02 [PMID: 18172035] (IHC-P, Mouse)
• Santos SJ, Haslam SZ, Conrad SE Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland. Endocrinology, 2007-09-20;149(1):329-38. 2007-09-20 [PMID: 17884938] (IHC-P, Mouse)
• Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance. J. Immunol., 2003-03-01;170(5):2655-62. 2003-03-01 [PMID: 12594294] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: TNFSF11
• Uniprot:
  • O35235()