TRANCE/TNFSF11/RANK L Antibody [Unconjugated]


In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent more
MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin more

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Reactivity MuSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)

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TRANCE/TNFSF11/RANK L Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L
Accession # O35235
Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.02-0.06 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse TRANCE/TNFSF11/RANK L Antibody (Catalog # AF462)
ELISA Detection: Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462)
Standard: Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)
Read Publications using
AF462 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody [Unconjugated]

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (AF462)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 7 applications: ELISA Development, ICC, IHC, IHC-P, Neutralization, Proximity Ligation Assay, Western Blot.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using AF462 Applications Species
Yamamoto, T;Abe, M;Hongo, H;Maruoka, H;Yoshino, H;Haraguchi-Kitakamae, M;Udagawa, N;Li, M;Amizuka, N;Hasegawa, T; Differential osteoblastic activity in primary metaphyseal trabecular and secondary trabeculae of c-fos deficient mice Journal of oral biosciences 2023-08-16 [PMID: 37595744] (IHC, Mouse) IHC Mouse
ZZ Lai, WJ Zhou, JW Shi, YH Meng, JN Wu, JF Ye, T Peng, CE Xu, MQ Li RANKL up-regulated by progesterone aggravates lipopolysaccharide-induced acute lung injury during pregnancy Journal of reproductive immunology, 2022-12-16;155(0):103788. 2022-12-16 [PMID: 36580846] (Neutralization, Mouse) Neutralization Mouse
S Sonoda, S Murata, H Yamaza, R Yuniartha, J Fujiyoshi, K Yoshimaru, T Matsuura, Y Oda, S Ohga, T Tajiri, T Taguchi, T Yamaza Targeting hepatic oxidative stress rescues bone loss in liver fibrosis Molecular Metabolism, 2022-09-13;66(0):101599. 2022-09-13 [PMID: 36113772] (Neutralization, Mouse) Neutralization Mouse
MM Szwarc, L Hai, VK Maurya, K Rajapakshe, D Perera, MM Ittmann, Q Mo, Y Lin, ML Bettini, C Coarfa, JP Lydon Histopathologic and transcriptomic phenotypes of a conditional RANKL transgenic mouse thymus Cytokine, 2022-09-11;160(0):156022. 2022-09-11 [PMID: 36099756] (IHC, Western Blot, Transgenic Mouse) IHC, Western Blot Transgenic Mouse
SH Lee, YHY Yap, CL Lim, ARE Woo, VCL Lin Activation function 1 of progesterone receptor is required for mammary development and regulation of RANKL during pregnancy Scientific Reports, 2022-07-19;12(1):12286. 2022-07-19 [PMID: 35854046] (IHC, Mouse) IHC Mouse
M Inoue, Y Nagai-Yosh, R Yamasaki, T Kawamoto, T Nishihara, W Ariyoshi Mechanisms involved in suppression of osteoclast supportive activity by transforming growth factor-beta1 via the ubiquitin-proteasome system PLoS ONE, 2022-02-23;17(2):e0262612. 2022-02-23 [PMID: 35196318] (Western Blot, Mouse) Western Blot Mouse
D Li, M San, J Zhang, A Yang, W Xie, Y Chen, X Lu, Y Zhang, M Zhao, X Feng, Y Zheng Oxytocin receptor induces mammary tumorigenesis through prolactin/p-STAT5 pathway Cell Death & Disease, 2021-06-07;12(6):588. 2021-06-07 [PMID: 34099636] (Western Blot, Mouse) Western Blot Mouse
HN Kim, J Xiong, RS MacLeod, S Iyer, Y Fujiwara, KM Cawley, L Han, Y He, JD Thostenson, E Ferreira, RL Jilka, D Zhou, M Almeida, CA O'Brien Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence JCI Insight, 2020-10-02;0(0):. 2020-10-02 [PMID: 32870816] (Western Blot, Mouse) Western Blot Mouse
M Li, D Xu Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site Proc. Natl. Acad. Sci. U.S.A., 2020-07-07;0(0):. 2020-07-07 [PMID: 32636266] (ICC, Proximity Ligation Assay, Mouse) ICC, Proximity Ligation Assay Mouse
R Pascual, J Martín, F Salvador, O Reina, V Chanes, A Millanes-R, C Suñer, G Fernández-, A Bartomeu, YS Huang, RR Gomis, R Méndez The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer Sci Adv, 2020-05-15;6(20):eaax3868. 2020-05-15 [PMID: 32440535] (IHC, Human) IHC Human
Show All 25 Publications.

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol TNFSF11