TRANCE/TNFSF11/RANK L Antibody (685809)

Western blot shows lysates of human primary differentiated CD4+Th2 cells. PVDF Membrane was probed with 2 µg/mL of Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6263) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRANCE/TNFSF11/RANK L at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 685809
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TRANCE/TNFSF11/RANK L Antibody (685809) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
  Gly64-Asp245
  Accession # AAC51762
• Specificity: Detects human TRANCE/TNFSF11/RANK L in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) TRANCE and approximately 25% cross-reactivity with recombinant human (rh) LIGHT is observed. No cross-reactivity with rh4-1BB Ligand, rhAPRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhOX40 LIgand, rhTNF-alpha , rhTRAIL, rhTWEAK, or rhVEGI is observed.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (685809)

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family.  Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation.  Splicing variants produce three isoforms for human TRANCE.  Isoform 1 is the standard form.  In isoform 2, aa corresponding to 1-73 in isoform 1 are missing.  In isoform 3, aa 1-47 are missing.  Human TRANCE ECD is 83% identical to mouse TRANCE ECD.  TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid.  TRANCE plays a role in osteoclast differentiation and activation. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (MAB6263)(1)

Publication using MAB6263

• BJ Kwon, GM Seon, MH Lee, MA Koo, MS Kim, D Kim, JJ Han, D Kim, J Kim, JC Park Locally delivered ethyl-2,5-dihydroxybenzoate using 3D printed bone implant for promotion of bone regeneration in a osteoporotic animal model Eur Cell Mater, 2018-01-12;35(0):1-12. 2018-01-12 [PMID: 29327779] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: TNFSF11
• Uniprot:
  • AAC51762()