TRAIP Antibody (OTI2D4) - Azide and BSA Free

Western Blot: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAIP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAIP.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Human Kidney tissue using anti-TRAIP mouse monoclonal antibody.

Flow Cytometry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TRAIP antibody, and then analyzed by flow cytometry.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Human colon tissue using anti-TRAIP mouse monoclonal antibody.

Immunohistochemistry: TRAIP Antibody (OTI2D4) - Azide and BSA Free [NBP2-74580] - Staining of paraffin-embedded Human liver tissue using anti-TRAIP mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TRAIP Antibody (OTI2D4) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 67-313 of human TRAIP (NP_005870) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRAIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 53.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TRAIP Antibody (OTI2D4) - Azide and BSA Free

• RNF206TRIPRING finger protein 206
• TRAF interacting protein
• TRAF-interacting protein

Background

The TNF receptor-associated factor (TRAF) family is a group of cytoplasmic adapter proteins that link a wide variety of cell surface receptors including the TNF and IL-1 receptor (TRNFR and IL-1R) superfamily to diverse signaling cascades involved in differentiation, proliferation, activation and apoptosis. TRIP (TRAF-interacting protein) was identified in 1997 as a component of receptor-TRAF signaling complexes (Lee, 1997). TRIP (also known as TRAIP) has been shown to associate with TRNFR2 or CD30 signaling complexes through its interaction with TRAF proteins and inhibit TRAF2-mediated NF-kB activation (Lee, 1997; Regamey et al, 2003). The recruitment of different TRAFs and TRAF associated proteins like TRIP to receptor complexes can help regulate and provide specificity to the intracellular signals triggered by the cell surface receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAIP