Recombinant Human TIFA His Protein

Recombinant Human TIFA Protein [NBP1-99103]

• Reactivity: Hu
• Applications: IP, PAGE
• Concentration: 0.5 mg/ml

Recombinant Human TIFA His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-184 of Human TIFA
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTSFED ADTEETVTCL QMTVYHPGQL QCGIFQSISF NREKLPSSEV VKFGRNSNIC HYTFQDKQVS RVQFSLQLFK KFNSSVLSFE IKNMSKKTNL IVDSRELGYL NKMDLPYRCM VRFGEYQFLM EKEDGESLEF FETQFILSPR SLLQENNWPP HRPIPEYGTY SLCSSQSSSP TEMDENES

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TIFA
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • SDS-Page
• Application Notes: Use in IP reported in scientific literature (PMID:35089437) .
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TIFA His Protein

• Putative MAPK-activating protein PM14
• Putative NF-kappa-B-activating protein 20
• T2BPMGC20791
• T6BP
• TIFAA
• TRAF2 binding protein
• TRAF2-binding protein
• TRAF6 binding protein
• TRAF-interacting protein with a forkhead-associated domain
• TRAF-interacting protein with FHA domain-containing protein A
• TRAF-interacting protein with forkhead-associated domain

Background

TIFA, also known as TRAF-interacting protein with FHA domain-containing protein A, is an adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. This protein induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. Recombinant human TIFA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for TIFA Recombinant Protein (NBP1-99103)(2)

Publications using NBP1-99103

• Lim MCC, Maubach G, Birkl-Toeglhofer AM et al. A20 undermines alternative NF-kappa B activity and expression of anti-apoptotic genes in Helicobacter pylori infection Cellular and molecular life sciences : CMLS 2022-01-28 [PMID: 35089437] (IP)
• Maubach G, Lim MCC, Sokolova O Et al. TIFA has dual functions in Helicobacter pylori-induced classical and alternative NF- kappa B pathways EMBO reports 2021-07-30 [PMID: 34328245]

Bioinformatics

• Gene Symbol: TIFA