Immunogen | Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C Ala26-Ala221 Accession # O14798 |
Specificity | Detects human TRAIL R3/TNFRSF10C in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, or rhTRAIL R4 is observed. |
Source | N/A |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | TNFRSF10C |
Purity Statement | Protein A or G purified from ascites |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
|
Application Notes | ELISA Detection: Human TRAIL R3/TNFRSF10C Biotinylated Affinity Purified PAb (Catalog number BAF630) Standard: Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera (Catalog number 630-TR) |
|
Publications |
|
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration. |
Human TRAIL R3, also called DcR1 (decoy receptor 1), LIT, and TRID and designated TNFRSF10C, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.