TRAILR3/TNFRSF10C Antibody (90905) [Unconjugated]

TRAIL R3/TNFRSF10C was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human TRAIL R3/TNFRSF10C Monoclonal Antibody (Catalog # MAB6301) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Cap)
• Clone: 90905
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TRAILR3/TNFRSF10C Antibody (90905) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRAIL R3/TNFRSF10C
  Ala26-Ala221
  Accession # O14798
• Specificity: Detects human TRAIL R3/TNFRSF10C in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, or rhTRAIL R4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF10C
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human TRAIL R3/TNFRSF10C Biotinylated Affinity Purified PAb (Catalog number BAF630)
  Standard: Recombinant Human TRAIL R3/TNFRSF10C Fc Chimera (Catalog number 630-TR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAILR3/TNFRSF10C Antibody (90905) [Unconjugated]

• Antagonist decoy receptor for TRAIL/Apo-2L
• CD263 antigen
• CD263
• cytotoxic TRAIL receptor-3
• DcR1
• DCR1MGC149502
• Decoy receptor 1
• Decoy TRAIL receptor without death domain
• LITMGC149501
• Lymphocyte inhibitor of TRAIL
• TNFRSF10C
• TRAIL R3
• TRAIL receptor 3
• TRAIL receptor without an intracellular domain
• TRAILR3
• TRAIL-R3
• TRAILR3TNF-related apoptosis-inducing ligand receptor 3
• TRIDDCR1-TNFR
• tumor necrosis factor receptor superfamily member 10C
• tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain

Background

Human TRAIL R3, also called DcR1 (decoy receptor 1), LIT, and TRID and designated TNFRSF10C, is a glycosyl-phosphatidylinositol-linked membrane protein which binds TRAIL (Apo2 Ligand) with high affinity. TRAIL R3 has the TRAIL-binding extracellular cysteine-rich domains but lacks the intracellular signalling domain. As a result, binding of TRAIL to TRAIL R3 does not transduce an apoptosis signal. Expression of TRAIL R3 has been shown to protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-induced apoptosis. A second TRAIL decoy receptor, which binds TRAIL with high-affinity but antagonizes TRAIL-induced apoptosis, named TRAIL R4, DcR2 or TRUNDD, has also been reported. The human soluble TRAIL R3/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

1. Sheridan, J.P. et al. (1997) Science 277:818.
2. Golstein, P. (1997) Curr. Biol. 7:R750.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAILR3/TNFRSF10C Antibody (MAB6301)(1)

Publication using MAB6301

• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009;87(12):1814-20. 2009 [PMID: 19543058] (Antibody Array Development, Human)

Bioinformatics

• Gene Symbol: TNFRSF10C
• Uniprot:
  • O14798()