Fas Ligand/TNFSF6 Antibody (100419) [Unconjugated]


HEK293 human embryonic kidney cell line transfected with (A) Fas Ligand/TNFSF6 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human Fas Ligand/TNFSF6 Monoclonal Antibody (Catalog # MAB126) followed ...read more
In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050), Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induces cytotoxicity in the Jurkat human ...read more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, B/N, ELISA(Cap)

Order Details

Fas Ligand/TNFSF6 Antibody (100419) [Unconjugated] Summary

Chinese hamster ovary cell line CHO-derived recombinant human Fas Ligand/TNFSF6
Accession # P48023
Detects human Fas Ligand/TNFSF6 in ELISAs.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 1-5 ng/mL
Application Notes
ELISA Capture: Human Fas Ligand/TNFSF6 Antibody (Catalog # MAB126)
ELISA Detection: Human Fas Ligand/TNFSF6 Biotinylated Antibody (Catalog # BAF126)
Standard: Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL)
Read Publications using
MAB126 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fas Ligand/TNFSF6 Antibody (100419) [Unconjugated]

  • apoptosis (APO-1) antigen ligand 1
  • Apoptosis antigen ligand
  • APT1LG1CD95L
  • APTL
  • CD178 antigen
  • CD178
  • CD95L
  • CD95-L
  • Fas antigen ligand
  • Fas ligand (TNF superfamily, member 6)
  • Fas Ligand
  • FASLCD95 ligand
  • TNFSF6
  • TNFSF6FasL
  • tumor necrosis factor (ligand) superfamily, member 6
  • tumor necrosis factor ligand superfamily member 6


Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain (2). Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Both mouse and human Fas Ligand are active on mouse and human cells (2, 3). Fas Ligand is expressed on the cell surface as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta , however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).
  1. Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
  2. Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
  3. Seino, K-I. et al. (1998) J. Immunol. 161:4484.
  4. Suda, T. et al. (1993) Cell 75:1169.
  5. Pitti, R.M. et al. (1998) Nature 396:699.
  6. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
  7. Tanaka, M. et al. (1998) Nature Med. 4:31.
  8. Chen, J-J. et al. (1998) Science 282:1714.
  9. Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. USA 97:1707.
  10. Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
  11. Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
  12. Takahashi, T. et al. (1994) Cell 76:969.
  13. Lynch, D.H. et al. (1994) Immunity 1:131.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fas Ligand/TNFSF6 Antibody (MAB126)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Functional Assay, Neutralization.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MAB126 Applications Species
S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022;13(1):3121. 2022 [PMID: 35660734] (Neutralization, Human) Neutralization Human
O Kajikawa, R Herrero, YH Chow, CF Hung, G Matute-Bel The bioactivity of soluble Fas ligand is modulated by key amino acids of its stalk region PLoS ONE, 2021;16(6):e0253260. 2021 [PMID: 34138914] (Functional Assay, Human) Functional Assay Human
B Liu, Y Zhang, Y Fan, S Wang, Z Li, M Deng, C Li, J Wang, R Ma, X Wang, Y Wang, L Xu, K Hou, X Che, Y Liu, X Qu Leucine-rich repeat neuronal protein-1 suppresses apoptosis of gastric cancer cells through regulation of Fas/FasL Cancer Sci., 2019;110(7):2145-2155. 2019 [PMID: 31087525] (Neutralization, Human) Neutralization Human
M Idelson, R Alper, A Obolensky, N Yachimovic, J Rachmilewi, A Ejzenberg, E Beider, E Banin, B Reubinoff Immunological Properties of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Stem Cell Reports, 2018;0(0):. 2018 [PMID: 30122442] (Neutralization, Human) Neutralization Human
M Kim, S Pyo, CH Kang, CO Lee, HK Lee, SU Choi, CH Park Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer PLoS ONE, 2018;13(6):e0198347. 2018 [PMID: 29874279] (Neutralization, Human) Neutralization Human
S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017;19(1):97. 2017 [PMID: 28511719] (Neutralization, Human) Neutralization Human
N Köstlin, M Vogelmann, B Spring, J Schwarz, J Feucht, C Härtel, TW Orlikowsky, CF Poets, C Gille Granulocytic myeloid derived suppressor cells from human cord blood modulate T-helper-cell response towards an anti-inflammatory phenotype Immunology, 2017;0(0):. 2017 [PMID: 28464218] (Neutralization, Human) Neutralization Human
MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways Cell Death Differ, 2017;0(0):. 2017 [PMID: 28106882] (Neutralization, Human) Neutralization Human
ME Guicciardi, A Krishnan, SF Bronk, P Hirsova, TS Griffith, GJ Gores Biliary tract instillation of a SMAC mimetic induces TRAIL-dependent acute sclerosing cholangitis-like injury in mice Cell Death Dis, 2017;8(1):e2535. 2017 [PMID: 28055006] (Neutralization, Human) Neutralization Human
Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice. PLoS ONE, 2015;10(8):e0135999. 2015 [PMID: 26285203] (Neutralization, Human) Neutralization Human
Show All 15 Publications.

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Gene Symbol FASLG