TRAILR2/TNFRSF10B Antibody - Azide and BSA Free

Western Blot: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAIL R2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image using the standard format of this product.

Immunocytochemistry/Immunofluorescence: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TRAIL R2 (H76) at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.

Immunohistochemistry-Paraffin: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in the Purkinje cell layer. Image using the standard format of this product.

Western Blot: TRAILR2/TNFRSF10B Antibody - Azide Free [NB100-55744] - Analysis of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL R2/TNFRSF10B at 5 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, MS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

TRAILR2/TNFRSF10B Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TRAILR2/TNFRSF10B Antibody - Azide and BSA Free (NB100-56618) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-TRAILR2/TNFRSF10B Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Rabbit anti-DR5 polyclonal antibody was raised against a peptide corresponding to amino acids 388 to 407 (isoform 2) of human DR5 precursor (1,2). The same sequence is found in isoform 1 at amino acids 417-436.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFRSF10B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy reported in scientific literature (PMID 16426839)
  • Flow (Cell Surface) reported in scientific literature (PMID 16426839)
  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 5 ug/ml
• Control:

  Jurkat Whole Cell Lysate
  TRAILR2/TNFRSF10B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TRAILR2/TNFRSF10B Antibody - Azide and BSA Free

• CD262 antigen
• CD262
• death domain containing receptor for TRAIL/Apo-2L
• Death receptor 5
• DR5
• DR5TRICK2B
• Fas-like protein
• KILLER/DR5
• KILLERapoptosis inducing protein TRICK2A/2B
• TNF-related apoptosis-inducing ligand receptor 2
• TNFRSF10B
• TRAIL R2
• TRAIL receptor 2
• TRAILR2
• TRAIL-R2apoptosis inducing receptor TRAIL-R2
• TRAILR2cytotoxic TRAIL receptor-2
• TRICK2
• TRICK2A
• TRICKB
• tumor necrosis factor receptor superfamily member 10B
• tumor necrosis factor receptor superfamily, member 10b
• tumor necrosis factor receptor-like protein ZTNFR9
• ZTNFR9

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAILR2/TNFRSF10B Antibody (NB100-55744)(19)

Publications using NB100-55744

• Xue M, Ge Y et al Apoptosis is induced by docosahexaenoic acid in breast cancer cells via death receptor and mitochondria-mediated pathways Mol Med Rep 2017-06-07 [PMID: 28586001] (WB, WB, Human)
  
  Details:
  Citation using the Azide Free version of this antibody.
• Wang S, Ren W, Liu J et al. TRAIL and doxorubicin combination induces proapoptotic and antiangiogenic effects in soft tissue sarcoma in vivo. Clin Cancer Res. 2010-05-01 [PMID: 20406839] (IHC-P, Human)
  
  Details:
  DR5/TRAILR2 (IMG-120A): IHC (paraffin), Human STS cell lines HT1080 and SkLMS1, Fig 1 and 2.
• Younes M, Georgakis GV, Rahmani M et al. Functional expression of TRAIL receptors TRAIL-R1 and TRAIL-R2 in esophageal adenocarcinoma. Eur J Cancer. 2006-03-01 [PMID: 16426839] (IHC-P, Flow-CS, WB)
  
  Details:
  IHC-paraffin: formalin-fixed, paraffin-embedded tissue sections and cell line), (WB: cell lines), (Flow-cell surface: cell lines).
• Abdelrahim M, Newman K, Vanderlaag K et al. 3,3'-diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5. Carcinogenesis. 2006-04-01 [PMID: 16332727] (WB)
  
  Details:
  ATF6 (IMG-273) 2. DR5 (IMG-120) [WB, Fig.4 and Fig. 6 (Panc-28 cells)].
• Georgakis GV, Li Y, Humphreys R et al. Activity of selective fully human agonistic antibodies to the TRAIL death receptors TRAIL-R1 and TRAIL-R2 in primary and cultured lymphoma cells: induction of apoptosis and enhancement of doxorubicin- and bortezomib-induced cell death. Br J Haematol. 2005-08-01 [PMID: 16098063] (ICC/IF)
  
  Details:
  ICC: cytospins.
• Zou W, Liu X, Yue P et al. c-Jun NH2-terminal kinase-mediated up-regulation of death receptor 5 contributes to induction of apoptosis by the novel synthetic triterpenoid methyl-2-cyano-3,12-dioxooleana-1, 9-dien-28-oate in human lung cancer cells. Cancer Res. 2004-10-15 [PMID: 15492284]
• Fan QL, Zou WY, Song LH et al. Synergistic antitumor activity of TRAIL combined with chemotherapeutic agents in A549 cell lines in vitro and in vivo. Cancer Chemother Pharmacol. 2005-02-01 [PMID: 15290100]
• Jin Z, McDonald ER 3rd, Dicker DT et al. Deficient tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor transport to the cell surface in human colon cancer cells selected for resistance to TRAIL-induced apoptosis. J Biol Chem [PMID: 15155747]
  
  Details:
  Used the Azide Free form of this antibody.
• Dandrea T, Hellmold H, Jonsson C et al. The transcriptosomal response of human A549 lung cells to a hydrogen peroxide-generating system: relationship to DNA damage, cell cycle arrest, and caspase activation. Free Radic Biol Med [PMID: 15019973]
  
  Details:
  Used the Azide Free form of this antibody.
• Kim K, Nakagawa H, Fei P et al. Targeting Bcl-xL in esophageal squamous cancer to sensitize to chemotherapy plus TRAIL-induced apoptosis while normal epithelial cells are protected by blockade of caspase 9. Cell Death Differ [PMID: 14752513]
  
  Details:
  Used the Azide Free form of this antibody.
• Koyama S. Flow cytometric measurement of tumor necrosis factor-related apoptosis-inducing ligand and its receptors in gastric epithelium and infiltrating mucosal lymphocytes in Helicobacter pylori-associated gastritis. J Gastroenterol Hepatol. 2003-07-01 [PMID: 12795746] (FLOW)
• Kandasamy K, Srinivasula SM, Alnemri ES et al. Involvement of proapoptotic molecules Bax and Bak in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced mitochondrial disruption and apoptosis: differential regulation of cytochrome c and Smac/DIABLO release. Cancer Res [PMID: 12670926]
  
  Details:
  Used the Azide Free form of this antibody.
• Eid MA, Lewis RW, Kumar MV. Mifepristone pretreatment overcomes resistance of prostate cancer cells to tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL). Mol Cancer Ther. [PMID: 12492116]
  
  Details:
  Citation using the Azide Free form of this antibody.
• Zhang HG, Xie J, Xu L et al. Hepatic DR5 induces apoptosis and limits adenovirus gene therapy product expression in the liver. J Virol. 2002-06-01 [PMID: 11991997]
• Koyama S, Koike N, Adachi S. Expression of TNF-related apoptosis-inducing ligand (TRAIL) and its receptors in gastric carcinoma and tumor-infiltrating lymphocytes: a possible mechanism of immune evasion of the tumor. J Cancer Res Clin Oncol [PMID: 11862476]
  
  Details:
  Used the Azide Free form of this antibody.
• Burns TF, Bernhard EJ, El-Deiry WS. Tissue specific expression of p53 target genes suggests a key role for KILLER/DR5 in p53-dependent apoptosis in vivo. Oncogene. 2001-08-02 [PMID: 11498783]
• Cuello M, Ettenberg SA, Clark AS et al. Down-regulation of the erbB-2 receptor by trastuzumab (herceptin) enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in breast and ovarian cancer cell lines that overexpress erbB-2. Cancer Res [PMID: 11406568]
  
  Details:
  Used the Azide Free form of this antibody.
• Basile JR, Zacny V, Munger K. The cytokines tumor necrosis factor-alpha (TNF-alpha ) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papillomavirus-16 E7 oncoprotein. J Biol Chem. 2001-06-22 [PMID: 11306566]
  
  Details:
  WB DR5/Apo2/TRAIL-R2/TRAILR2/TRICK2/KILLER (IMG-120A) and DcR2/TRAIL-R4/TRUNDD (IMG-121). Human keratinocytes (HFK) cells expressing HPV-16 EF (human papillomavirus), Fig 6.
• Kim K, Fisher MJ, Xu SQ, el-Deiry WS. Molecular determinants of response to TRAIL in killing of normal and cancer cells. Clin Cancer Res. 2000-02-01 [PMID: 10690508]

Bioinformatics

• Gene Symbol: TNFRSF10B