TRAF-6 Antibody

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Western Blot: TRAF-6 Antibody [NBP1-33357] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRAF-6 Antibody [NBP1-33357] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRAF6 protein stained by TRAF6 antibody Internal diluted at 1:100. Red: ...read more
Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded rat brain. TRAF6 antibody [N2C2], Internal diluted at 1:500.
Western Blot: TRAF-6 Antibody [NBP1-33357] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:1000
Western Blot: TRAF-6 Antibody [NBP1-33357] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:1000
Western Blot: TRAF-6 Antibody [NBP1-33357] - TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis. A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5 % SDS-PAGE TRAF6 ...read more
Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded 59T xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded mouse lung diluted at 1:500.
Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.17 mg/ml

Order Details

TRAF-6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Porcine (90%), Bovine (90%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAF6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.17 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRAF-6 Antibody

  • E3 ubiquitin-protein ligase TRAF6
  • EC 6.3.2.-
  • Interleukin-1 signal transducer
  • RING finger protein 85
  • RNF85MGC:3310
  • TNF receptor-associated factor 6
  • TRAF6
  • TRAF-6

Background

TRAFs (Tumor necrosis factor receptor-associated factors) are cytoplasmic adaptor proteins that associate with tumor necrosis factor receptors (TNFR), thus mediating tumor necrosis factor (TNF) induced signaling. The TRAF family consists of six members (TRAF1-6).1-3 TRAF proteins play an important role in regulating cellular death and cellular response to stress by mediating stimulation of AP-1 family through activation of MAP kinases, including JNK/SAPKs, ERKs and p38s.4 They are also involved in the regulation of inflammatory and anti-apoptotic responses through activation of the transcription factors of NF-kB.5 TRAF6 possesses several binding motifs in CD40, TRANCE-R and IRAK suggesting a crucial role in TRANCE-R signaling. It is involved in the activation of the anti-apoptotic serine/threonine kinase Akt/PKB through TRANCE-R.6 Due to its specific receptor-binding TRAF-C domain, TRAF6 is the only member of the TRAF family that also participates in signal transduction of the interlukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily.1,7,8 TRAF6 plays a crucial role in adaptive immunity, innate immunity, bone homeostasis, perinatal and postnatal survival, and cytokine signaling.9 Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Reacts with the human and mouse TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids). Reactivity has been confirmed with human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates.Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRAF6
Entrez