TRAF-6 Antibody


Western Blot: TRAF-6 Antibody [NBP1-33357] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRAF-6 Antibody [NBP1-33357] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRAF6 protein stained by TRAF6 antibody Internal diluted at 1:100. Red: more
Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded rat brain. TRAF6 antibody [N2C2], Internal diluted at 1:500.
Western Blot: TRAF-6 Antibody [NBP1-33357] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:1000
Western Blot: TRAF-6 Antibody [NBP1-33357] - Sample (30 ug of whole cell lysate) A: A549 B: HeLa C: HepG2 7.5% SDS PAGE diluted at 1:1000
Western Blot: TRAF-6 Antibody [NBP1-33357] - TRAF6 antibody [N2C2], Internal detects TRAF6 protein by western blot analysis. A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5 % SDS-PAGE TRAF6 more
Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded 59T xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Paraffin-embedded mouse lung diluted at 1:500.
Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP

Order Details

TRAF-6 Antibody Summary

Recombinant protein encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.
Predicted Species
Porcine (90%), Rhesus Macaque (97%), Bovine (90%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified

Alternate Names for TRAF-6 Antibody

  • E3 ubiquitin-protein ligase TRAF6
  • EC 6.3.2.-
  • Interleukin-1 signal transducer
  • RING finger protein 85
  • RNF85MGC:3310
  • TNF receptor-associated factor 6
  • TRAF6
  • TRAF-6


TRAFs (Tumor necrosis factor receptor-associated factors) are cytoplasmic adaptor proteins that associate with tumor necrosis factor receptors (TNFR), thus mediating tumor necrosis factor (TNF) induced signaling. The TRAF family consists of six members (TRAF1-6).1-3 TRAF proteins play an important role in regulating cellular death and cellular response to stress by mediating stimulation of AP-1 family through activation of MAP kinases, including JNK/SAPKs, ERKs and p38s.4 They are also involved in the regulation of inflammatory and anti-apoptotic responses through activation of the transcription factors of NF-kB.5 TRAF6 possesses several binding motifs in CD40, TRANCE-R and IRAK suggesting a crucial role in TRANCE-R signaling. It is involved in the activation of the anti-apoptotic serine/threonine kinase Akt/PKB through TRANCE-R.6 Due to its specific receptor-binding TRAF-C domain, TRAF6 is the only member of the TRAF family that also participates in signal transduction of the interlukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily.1,7,8 TRAF6 plays a crucial role in adaptive immunity, innate immunity, bone homeostasis, perinatal and postnatal survival, and cytokine signaling.9 Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Reacts with the human and mouse TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids). Reactivity has been confirmed with human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates.Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
Species: Hu, Mu, Rt
Applications: WB, IP
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB
Species: Hu, Ca
Applications: WB, B/N, Flow, ICC/IF, IHC-P, IP, Flow-CS
Species: Hu
Applications: Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, ICC, KO

Publications for TRAF-6 Antibody (NBP1-33357) (0)

There are no publications for TRAF-6 Antibody (NBP1-33357).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TRAF-6 Antibody (NBP1-33357) (0)

There are no reviews for TRAF-6 Antibody (NBP1-33357). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRAF-6 Antibody (NBP1-33357) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional TRAF-6 Products

Bioinformatics Tool for TRAF-6 Antibody (NBP1-33357)

Discover related pathways, diseases and genes to TRAF-6 Antibody (NBP1-33357). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRAF-6 Antibody (NBP1-33357)

Discover more about diseases related to TRAF-6 Antibody (NBP1-33357).

Pathways for TRAF-6 Antibody (NBP1-33357)

View related products by pathway.

PTMs for TRAF-6 Antibody (NBP1-33357)

Learn more about PTMs related to TRAF-6 Antibody (NBP1-33357).

Research Areas for TRAF-6 Antibody (NBP1-33357)

Find related products by research area.

Blogs on TRAF-6

There are no specific blogs for TRAF-6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our TRAF-6 Antibody and receive a gift card or discount.


Gene Symbol TRAF6