TRAF-5 Antibody (55A219)

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Western Blot: TRAF-5 Antibody (55A219) [NB600-1107] - Analysis of TRAF-5 using this antibody at 2 ug/ml dilution against 10 ug of HeLa cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
55A219
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

TRAF-5 Antibody (55A219) Summary

Immunogen
Anti-TRAF5 monoclonal antibody was raised against a fusion protein corresponding to amino acids 77 to 186 of human TRAF5.
Localization
Cytoplasmic (probable)
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
TRAF5
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
Application Notes
This antibody can be used in Western Blot where a 66 kDa band is seen (predicted molecular weight 66.8 kDa).
Positive Control
HeLa Lysate (NB800-PC1)
TRAF-5 Lysate (NBL1-17245)
TRAF-5 Lysate (NBP2-66289)

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for TRAF-5 Antibody (55A219)

  • MGC:39780
  • RNF84
  • RNF84RING finger protein 84
  • TNF receptor-associated factor 5
  • TRAF5
  • TRAF-5

Background

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor. Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymhocyte activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TRAF-5 Antibody (NB600-1107). (Showing 1 - 1 of 1 FAQ).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-1107AF405
Alexa Fluor 488 Labeled NB600-1107AF488
Alexa Fluor 647 Labeled NB600-1107AF647
Alexa Fluor 700 Labeled NB600-1107AF700
Biotin Labeled NB600-1107B
DyLight 350 Labeled NB600-1107UV
DyLight 405 Labeled NB600-1107V
DyLight 488 Labeled NB600-1107G
DyLight 550 Labeled NB600-1107R
DyLight 650 Labeled NB600-1107C
DyLight 680 Labeled NB600-1107FR
DyLight 755 Labeled NB600-1107IR
HRP Labeled NB600-1107H

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Blogs on TRAF-5

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Bioinformatics

Gene Symbol TRAF5
Entrez
Uniprot