TRAF-5 Antibody (55A219) - Azide and BSA Free

Western Blot: TRAF-5 Antibody (55A219) - Azide and BSA Free [NBP2-80988] - Analysis of TRAF-5 using this antibody at 2 ug/ml dilution against 10 ug of HeLa cell lysate. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB
• Clone: 55A219
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

TRAF-5 Antibody (55A219) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TRAF-5 Antibody (55A219) - Azide and BSA Free (NB600-1107) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Anti-TRAF5 monoclonal antibody was raised against a fusion protein corresponding to amino acids 77 to 186 of human TRAF5.
• Localization: Cytoplasmic (probable)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRAF5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: A 66 kDa band is seen in Western Blot (predicted molecular weight 66.8 kDa).

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TRAF-5 Antibody (55A219) - Azide and BSA Free

• MGC:39780
• RNF84
• RNF84RING finger protein 84
• TNF receptor-associated factor 5
• TRAF5
• TRAF-5

Background

Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymhocyte activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAF5