TRAF-2 Antibody

Immunohistochemistry-Paraffin: TRAF-2 Antibody - Unpurified [NB100-56174] - Formalin-fixed, paraffin-embedded mouse pancreas stained for TRAF2 expression using this antibody at 1:2000. Hematoxylin eosin counterstain. A1 is a high magnification of A. Expression of TRAF2 is seen in the pancreatic islets.

Immunohistochemistry-Paraffin: TRAF-2 Antibody - Unpurified [NB100-56174] - Formalin-fixed, paraffin-embedded mouse testis/epidymis stained for TRAF2 expression using this antibody at 1:2000. Hematoxylin eosin counterstain.

Product Discontinued

TRAF-2 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 250-271(CSFLEAQASPGTLNQVGPELLQR) of mouse TRAF2 was used as immunogen, GenBank no. NP_033448.2.
• Specificity: The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRAF2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for TRAF-2 Antibody

• E3 ubiquitin-protein ligase TRAF2
• EC 6.3.2.-
• MGC:45012
• TNF receptor-associated factor 2
• TRAF2
• TRAF-2
• TRAP3
• TRAP3TRAP
• tumor necrosis factor type 2 receptor associated protein 3
• Tumor necrosis factor type 2 receptor-associated protein 3

Background

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor super families and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. NB100-56174 recognizes TRAF2. TRAF2 is typically described as a 501 amino acid protein, GenBank no. NP_033448.2. However, various TRAF2 isoforms have been described. Therefore, the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed, please see the Application Notes for additional details.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAF2
• Entrez:
  • 22030(Mouse)