TPR Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: TPR Antibody [NB100-2867] - NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-TPR used at a dilution of 1:250. Detection: anti-rabbit IgG-FITC conjugated used at ...read more
Immunohistochemistry: TPR Antibody [NB100-2867] - Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti- TPR used at a dilution of 1:100 (2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-TPR used at a dilution of 1:100. Detection: DAB staining using anti-Rabbit IHC antibody ...read more
Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-TPR used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC ...read more
Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-TPR used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC ...read more
Immunohistochemistry: TPR Antibody [NB100-2867] - Sample: FFPE section of human testis. Antibody: Affinity purified rabbit anti- TPR used at a dilution of 1:100 (2ug/ml). Detection: DAB
Immunoprecipitation: TPR Antibody [NB100-2867] - Detection of Human TPR on HeLa whole cell lysate using NB100-2867. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2866.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

TPR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2300 and the C-terminus (residue 2349) of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:25 - 1:250
  • Immunohistochemistry 1:50 to 1:500
  • Immunohistochemistry-Paraffin 1:50 to 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
For IHC: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. For ICC/IF: Permeabilization with Triton-X 100 is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TPR Antibody

  • nuclear pore complex-associated protein TPR
  • nucleoprotein TPR
  • translocated promoter region (to activated MET oncogene)
  • tumor potentiating region

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPR
Entrez
Uniprot