TPR Antibody

NBF-fixed asynchronous HeLa cells. Affinity purified rabbit anti-TPR used at a dilution of 1:250. Detection: anti-rabbit IgG-FITC conjugated used at a dilution of 1:100.

Immunohistochemistry-Paraffin: TPR Antibody [NB100-2867] - Human testis. Antibody: Affinity purified rabbit anti- TPR used at a dilution of 1:100 (2ug/ml). Detection: DAB

Immunoprecipitation: TPR Antibody [NB100-2867] - Detection of Human TPR on HeLa whole cell lysate using NB100-2867. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2866.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TPR Antibody Summary

• Description: Novus Biologicals Rabbit TPR Antibody (NB100-2867) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-TPR Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2300 and the C-terminus (residue 2349) of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50 to 1:500
  • Immunohistochemistry-Paraffin 1:50 to 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: For IHC: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. For ICC/IF: Permeabilization with Triton-X 100 is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TPR Antibody

• nuclear pore complex-associated protein TPR
• nucleoprotein TPR
• translocated promoter region (to activated MET oncogene)
• tumor potentiating region

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPR Antibody (NB100-2867)(1)

Publication using NB100-2867

• Almeida AC, Soares-de-Oliveira J, Drpic D et al. Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals Cell reports 2022-04-05 [PMID: 35385739]

Review for TPR Antibody (NB100-2867) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot WB Human 07/19/2018

Summary

• Application: Western Blot
• Sample Tested: Nuclear fraction from H441
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: TPR
• Entrez:
  • 7175(Human)
• Uniprot:
  • P12270(Human)