TOP2B Antibody

Western Blot: TOP2B Antibody [NBP2-20684] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Topoisomerase II beta antibody [C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-20684] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse brain. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: TOP2B Antibody [NBP2-20684] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:3000.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Ovarian cancer, using Topoisomerase II beta antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse brain. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: TOP2B Antibody [NBP2-20684] - Mouse testis. Topoisomerase II beta stained by Topoisomerase II beta antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunoprecipitation: TOP2B Antibody [NBP2-20684] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 ug of Topoisomerase II beta antibody 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TOP2B Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human TOP2B. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus; nucleolus
• Predicted Species: Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOP2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 183 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TOP2B Antibody

• antigen MLAA-44
• DNA topoisomerase 2-beta
• DNA topoisomerase II beta
• DNA topoisomerase II, 180 kD
• DNA topoisomerase II, beta isozyme
• EC 5.99.1.3
• TOP2B
• top2beta
• TOPIIB
• topo II beta
• topoisomerase (DNA) II beta (180kD)
• topoisomerase (DNA) II beta 180kDa
• topoisomerase II beta
• topoisomerase IIb
• U937 associated antigen

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TOP2B
• Uniprot:
  • Q02880(Human)