RAR alpha/NR1B1 Antibody (763)

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Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, GPSpecies Glossary
Applications WB, IHC-Fr
Clone
763
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RAR alpha/NR1B1 Antibody (763) Summary

Immunogen
Peptide corresponding to amino acid residues from the N-terminal region of human retinoic acid receptor, alpha-isotype.
Specificity
Specific for the ~48k RAR-alpha isotype.
Predicted Species
Canine (100%), Bovine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RARA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry-Frozen
Application Notes
Western blot where a ~48 kDa band can be seen using rat brain hippocampal homogenate. (Tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24504337)
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB200-322 in the following applications:

Publications
Read Publication using
NB200-322 in the following applications:

Reactivity Notes

Tested in Human. Mouse reactivity reported in scientific literature (PMID: 24504337) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. It is anticipated that the antibody will also work with bovine, canine, and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RAR alpha/NR1B1 Antibody (763)

  • alpha polypeptide
  • NR1B1
  • RAR alpha
  • RARA
  • RAR-alpha
  • retinoic acid receptor, alpha

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Retinoic acid receptor alpha mRNA expression levels are significantly higher in Barrett's tissues compared with normal esophagus tissues (3). In addition, RAR alpha controls the action of all-trans retinoic acid in gastric cancer cells (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAR alpha/NR1B1 Antibody (NB200-322)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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Review for RAR alpha/NR1B1 Antibody (NB200-322) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB200-322:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot RAR alpha/NR1B1 NB200-322
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reviewed by:
Anonymous
WB Human 09/11/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from MOLT-4 cells, in lysis buffer
SpeciesHuman
CommentsThe antibody gives multiple bands like most other RARa antibodies I have tested but serves the purpose for WB. May not be ideal for ChIP.

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:500, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1min

Comments

CommentsThe antibody gives multiple bands like most other RARa antibodies I have tested but serves the purpose for WB. May not be ideal for ChIP.

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FAQs for RAR alpha/NR1B1 Antibody (NB200-322) (0)

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Secondary Antibodies

 

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Recent Reviews

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Anonymous
09/11/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RARA
Entrez
Uniprot