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RAR alpha/NR1B1 Antibody (763)

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Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.
Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, GPSpecies Glossary
Applications WB, IHC-Fr
Clone
763
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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RAR alpha/NR1B1 Antibody (763) Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Specificity
Specific for the ~48k RAR-alpha isotype.
Predicted Species
Canine (100%), Bovine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RARA
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry-Frozen
Application Notes
Western blot where a ~48 kDa band can be seen using rat brain hippocampal homogenate. (Tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24504337)
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB200-322 in the following applications:
Publications
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NB200-322 in the following applications:

Reactivity Notes

Tested in Human. Mouse reactivity reported in scientific literature (PMID: 24504337) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. It is anticipated that the antibody will also work with bovine, canine, and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RAR alpha/NR1B1 Antibody (763)

  • alpha polypeptide
  • NR1B1
  • RAR alpha
  • RARA
  • RAR-alpha
  • retinoic acid receptor, alpha

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Retinoic acid receptor alpha mRNA expression levels are significantly higher in Barrett's tissues compared with normal esophagus tissues (3). In addition, RAR alpha controls the action of all-trans retinoic acid in gastric cancer cells (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59787] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human stomach using 1:50 dilution of PML antibody. The signal was developed using HRP-labeled secondary with DAB reagent followed by hematoxylin counterstaining. This antibody generated an expected strong nuclear staining in most of the cells in the glandular as well as the connective tissue. The cells showed some cytoplasmic signal also which was much weaker than the nuclear staining.
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Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Top panel: Analysis of c-Fos expression in HeLa cells using NBP2-50037. Lane 1: HeLa cells were serum-starved for 36 hours. Lane 2: Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. NBP2-50037 recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel: Blot was stripped and probed with monoclonal antibody against GAPDH (NB300-221) used as loading control.
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RAR alpha/NR1B1
Antibody (763)
NB200-322
RAR alpha/NR1B1 Antibody (763)
Species: Hu, Mu, Rt, Bv, Ca, GP
Applications: WB, IHC-Fr

Publications for RAR alpha/NR1B1 Antibody (NB200-322)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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Review for RAR alpha/NR1B1 Antibody (NB200-322) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB200-322:
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Images Ratings Applications Species Date Details
Western Blot RAR alpha/NR1B1 NB200-322
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reviewed by:
Anonymous
 WB Human 09/11/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from MOLT-4 cells, in lysis buffer
SpeciesHuman
CommentsThe antibody gives multiple bands like most other RARa antibodies I have tested but serves the purpose for WB. May not be ideal for ChIP.

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:500, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1min

Comments

CommentsThe antibody gives multiple bands like most other RARa antibodies I have tested but serves the purpose for WB. May not be ideal for ChIP.

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Western Blot RAR alpha/NR1B1 NB200-322
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 Anonymous
09/11/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol RARA
Entrez
Uniprot