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RAR alpha/NR1B1 Antibody (763)

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Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.
Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, GpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clone
763
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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RAR alpha/NR1B1 Antibody (763) Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P10276
Specificity
Specific for the ~48k RAR-alpha isotype.
Predicted Species
Canine (100%), Guinea Pig (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RARA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:1000
Application Notes
Western blot where a ~48 kDa band can be seen using rat brain hippocampal homogenate. (Tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24504337). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:24504337).
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB200-322 in the following applications:
Publications
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NB200-322 in the following applications:

Reactivity Notes

Tested in Human. Mouse reactivity reported in scientific literature (PMID: 24504337) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. It is anticipated that the antibody will also work with bovine, canine, and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for RAR alpha/NR1B1 Antibody (763)

  • alpha polypeptide
  • NR1B1
  • RAR alpha
  • RARA
  • RAR-alpha
  • retinoic acid receptor, alpha

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Retinoic acid receptor alpha mRNA expression levels are significantly higher in Barrett's tissues compared with normal esophagus tissues (3). In addition, RAR alpha controls the action of all-trans retinoic acid in gastric cancer cells (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: RAR beta/NR1B2 Antibody [NBP1-81776] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RAR beta/NR1B2 Antibody [NBP1-81776] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: RAR beta/NR1B2 Antibody [NBP1-81776] - Staining of human pancreas shows weak nuclear positivity in exocrine glandular cells.
(1 Publication)
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Western Blot: PML Protein Antibody [NB100-59787] - PML-RARa is a HIF-I+-transcriptional co-activator. HEK-293 cells transfected with stable mutants of HIF-1I+-or HIF-2I+-and increasing concentrations of PML-RARI+-. Image collected and cropped by Citeab from the following publication (HIF factors cooperate with PML-RAR +/- to promote acute promyelocytic leukemia progression and relapse. EMBO Mol Med (2014)) licensed under a CC-BY licence.
Immunohistochemistry: PML Protein Antibody [NB100-59787] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunocytochemistry/Immunofluorescence: PML Protein Antibody - BSA Free [NB100-59787] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with PML Protein Antibody (NB100-59787) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
(1 Review)
(34 Publications)
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Western Blot: Retinoic Acid Receptor gamma Antibody [NBP2-15098] - 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Retinoic Acid Receptor gamma Antibody [NBP2-15098] - A. 30 ug A549 whole cell lysate/extract, B. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Species: Hu, Mu
Applications: WB
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Antibody (SY0297)
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Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
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[Unconjugated]
AF2944
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HEL 92.1.7 human erythroleukemic cell line, LNCaP human prostate cancer cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLZF at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for PLZF at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLZF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2944) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(15 Publications)
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c-Fos Antibody
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Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
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Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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(10 Publications)
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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
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Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(2 Reviews)
(6 Publications)
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Applications: IHC, IHC-P, IP, WB
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Immunohistochemistry-Paraffin: TMEFF2 Antibody (1F9) [NBP2-01863] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TMEFF2 mouse monoclonal antibody.
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TfR (Transferrin
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NB200-585
Western Blot: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Total protein from rat adrenal gland cells (PC12) and rat placenta was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-CD71 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Flow Cytometry: TfR (Transferrin R) Antibody (OX26) [NB200-585] - Staining of rat spleen cells with mouse anti rat CD71: RPE.
Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (OX26) [NB200-585] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti TfR/Transferrin R (OX26) NB200-585 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (red) at a 1:200 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.
(16 Publications)
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Thioredoxin
Reductase 1/TRXR1 Ant
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Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - Sample (30 ug of whole cell lysate)A: Molt-4 7. 5% SDS PAGE gel, diluted at 1:3000.
Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - 50 ug mouse brain lysate/extract 7. 5% SDS-PAGE TrxR1 antibody [N3C2], Internal dilution: 1:1000.
Western Blot: thioredoxin reductase 1 Antibody [NBP2-20619] - 50 ug Rat brain lysate/extract 7. 5% SDS-PAGE TrxR1 antibody [N3C2], Internal dilution: 1:1000.
(1 Publication)
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Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
(3 Publications)
Species: Hu, Mu
Applications: CHIP-SEQ, ChIP, IP, WB
VDR/NR1I1/Vitamin
D Receptor Antibody ( ...
NBP2-66778
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Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
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Antibody (WA-1)
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Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(16 Publications)
Species: Pm, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
FosB/G0S3 Antibody
(SN703)
NBP2-67710
Immunohistochemistry-Paraffin: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of paraffin-embedded mouse brain tissue using anti-FosB antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of paraffin-embedded rat spleen tissue using anti-FosB antibody. Counter stained with hematoxylin.
Western Blot: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of FosB on human liver lysates using anti-FosB antibody at 1/1,000 dilution.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
JunD [p Ser255]
Antibody (JJ08-21)
NBP2-67861
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
RAR alpha/NR1B1
Antibody (763)
NB200-322
RAR alpha/NR1B1 Antibody (763)
Species: Hu, Mu, Rt, Bv, Ca, Gp
Applications: WB, ICC/IF, IHC, IHC-Fr

Publications for RAR alpha/NR1B1 Antibody (NB200-322)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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Review for RAR alpha/NR1B1 Antibody (NB200-322) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB200-322:
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Western Blot RAR alpha/NR1B1 NB200-322
Enlarge 		3
reviewed by:
Costache Vasilescu
 Western Blot Human 09/11/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from MOLT-4 cells, in lysis buffer
SpeciesHuman

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Western Blot RAR alpha/NR1B1 NB200-322
Enlarge
 Costache Vasilescu
09/11/2014
Application: Western Blot
Species: Human
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Bioinformatics

Gene Symbol RARA
Entrez
Uniprot