Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining in human testis and kidney tissues using anti-TOP2A antibody. Corresponding TOP2A RNA-seq data are presented for the ...read more
Western Blot: TOP2A Antibody [NBP1-86928] - Analysis in human cell line RH-30.
Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.
This antibody was developed against Recombinant Protein corresponding to amino acids: DASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKG
Immunogen affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2), 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for TOP2A Antibody
DNA topoisomerase (ATP-hydrolyzing)
DNA topoisomerase 2-alpha
DNA topoisomerase II, 170 kD
DNA topoisomerase II, alpha isozyme
topoisomerase (DNA) II alpha (170kD)
topoisomerase (DNA) II alpha 170kDa
Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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